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ABSTRACT: Tea fresh leaves withering treatment at different times

PROVIDER: PRJNA1217791 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32208131_1.fastq.gz	Fastqsanger.gz
	SRR32208131_2.fastq.gz	Fastqsanger.gz
	SRR32208132_1.fastq.gz	Fastqsanger.gz
	SRR32208132_2.fastq.gz	Fastqsanger.gz
	SRR32208133_1.fastq.gz	Fastqsanger.gz

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Investigating Relationship between Plant Hormones and Important Substances in Harvested Tea Leaves Before Withering

Project description:Senescence is initiated immediately in harvested tea leaves, and leads to physiological and biochemical changes, and could affects the final tea products. In the present work, we investigated the relationship between hormones and critical components in harvested tea leaves before withering, changes in hormones including abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and critical components like catechins, theanine, and caffeine were analyzed. Significant changes in these substances were identified and ABA correlated with catechin in harvested tea leaves before withering. RNA-seq transcriptome analysis revealed dramatic differences between tea samples at 1 h and 2 h compared with those at 0 h. The patterns of these three critical components correlated with the expression profiles of differentially expressed genes (DEGs). Weighted correlation network analysis of co-expressed genes revealed that genes in the mediumpurple2 module correlated with ABA and catechins. The results of this study suggest that harvested tea leaves before withering undergo significant hormonal changes (ABA, JA, and SA) and ABA may participate in regulating catechin biosynthesis.

2020-01-31 | GSE143970 | GEO

Investigating the Relationships between Hormones and Critical Substances in Tea Leaves During Withering

Project description:To investigate the relationships between hormones and critical components in tea leaves during withering process, we detected the alterations of abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), catechins, theanine, and caffeine in tea leaves withered at different time points from 0 to 24 hours. The content of ABA increased from 0h to 9h and decreased thereafter and JA content continuous increased, however, SA content was no significantly changes during withering process. Except for gallocatechin (GC) and epicatechin (EC), the contents of other critical components were significantly reduced at 24h. Transcriptome analysis shown that compared with 0h, a total of 2,256, 3,654, and 1,275 differentially expressed genes (DEGs) were identified at 9h, 15h, and 24h, respectively. The pathways of“Phenylalanine, tyrosine and tryptophan biosynthesis”, and “Phenylalanine metabolism” involved in biosynthesis of catechins were enriched significantly with DEGs of all comparisons. Weighted correlation network analysis (WGCNA) of co-expression genes indicated that many of modules were correlated with a specific trait only, however, the darkolivegreen module were correlated with two traits ABA and theanine during withering process. Our study indicates that withering induced dramatic alteration of the gene transcription, hormones (ABA, JA, and SA) and important components, and ABA may regulate theanine matebolism during this process.

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Project description:Different degree of withering of tea

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Characterization of white tea metabolome: comparison against green and black tea by a nontargeted metabolomics approach

Project description:White tea is considered the least processed form of tea and is reported to have a series of potent bioactivities, such as antioxidant, anti-inflammatory, anti-mutagenic, and anti-cancer activities. However, the chemical composition of white tea and the dynamic changes of the metabolites during the manufacturing process are far from clear. In this study, we applied a nontargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to comprehensively profile the characteristic metabolites of white tea. There were significant differences in the content of amino acids, catechins, dimeric catechins, flavonol and flavone glycosides, and aroma precursors in white tea compared with green and black teas that were manufactured from the same fresh tea leaves. Furthermore, the dynamic changes of the metabolites in the tea samples with various withering durations of 0, 4, 8, 12, 16, 20, 24, 28, and 36 h were also profiled. To the best of our knowledge, this study offers the most comprehensive characterization of the metabolites and their changes in white tea.

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RAW macrophages: Control vs. LPS treated or Immunobiotic treated

Project description:The ability of Lactiplantibacillus plantarum LOC1 and LOC3, originally isolated from fresh tea leaves, to modulate the response of murine macrophages to the activation of Toll-like receptor 4 (TLR4) by the stimulation with lipopolysaccharide (LPS) was evaluated.

2022-10-22 | GSE213389 | GEO

dehydration and cell wall metabolism in tobacco (Nicotiana tabacum L.) leaves withering

Project description:Withering is an important biological process associated with dehydration and cell wall metabolism in postharvest plant organs during curing/processing and storage. However, the withering mechanisms involved in dehydration and cell wall metabolism in postharvest tobacco leaves remain elusive. Here, TMT-based quantitative proteomic analysis in tobacco leaves under different withering conditions was performed. In total, 496 differentially abundant proteins (DAPs) were identified. To elucidate the withering mechanism of tobacco leaves, 28 DAPs associated with cell wall metabolism were screened.

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Project description:The effects of withering of tea

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Investigating the Relationships between Hormones and Critical Substances in Tea Leaves During Withering

Project description:Investigating the Relationships between Hormones and Critical Substances in Tea Leaves During Withering

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Investigating Relationship between Plant Hormones and Important Substances in Harvested Tea Leaves Before Withering

Project description:Investigating Relationship between Plant Hormones and Important Substances in Harvested Tea Leaves Before Withering

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