Murshidia linstowi
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1217869 | ENA |
REPOSITORIES: ENA
Similar Datasets
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Project description:Efforts to implement effective assisted reproductive technologies (ART) to extricate the northern white rhinoceros (NWR; Ceratotherium simum cottoni) from extinction, could be unconventionally offset from studies carried out using the southern white rhinoceros (Ceratotherium simum simum) as a relative model species. The bi-directional communication and critical transport of regulatory molecules controlling follicular growth and oocyte development are in part mediated through extracellular vesicles (EVs), which encompass a highly conserved and advanced paracrine signaling mechanism important in shuttling unique cargo such as microRNAs (miRNAs). In this study, critical miRNAs for follicular development were identified, proposing novel approaches using EV-mediated miRNA to possibly improve the in vitro technologies outcome in a multitude of species.
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Project description:The crisis of biodiversity loss in the present era requires new tools for studying non-model organisms. Elephants are both an endangered species and excellent models for studying complex phenotypes including body size, social behavior, and longevity. Here we report here the first derivation of (Elephas maximus) induced pluripotent stem cells (emiPSCs). We achieved emiPSCs using two approaches: (1) a two-step process of chemical-media induction and colony selection followed by overexpression of elephant transcription factors; and (2) a one-step process with the same transgenes but without the chemical induction step and including an additional HRAS mutant transgene, HRASG12V. For both protocols, we inhibited TP53 expansion genes, which are hypothesized to confer unique cancer resistance in elephants. To confirm their reprogrammed state, we generated a functional genomics catalog of the modified cells with RNA-seq, ATAC-seq, and single-molecule direct RNA-sequencing. While these emiPSCs are transgene-dependent, we inactivated them to differentiate emiPSCs into all three germ layers via tri-lineage differentiation, embryoid body generation, and direct differentiation into putative cell types from all three layers. These methods will open new frontiers for cellular models of non-model organisms, including for genetic rescue and conservation.
2026-03-03 | GSE283468 | GEO
Project description:The crisis of biodiversity loss in the present era requires new tools for studying non-model organisms. Elephants are both an endangered species and excellent models for studying complex phenotypes including body size, social behavior, and longevity. Here we report here the first derivation of (Elephas maximus) induced pluripotent stem cells (emiPSCs). We achieved emiPSCs using two approaches: (1) a two-step process of chemical-media induction and colony selection followed by overexpression of elephant transcription factors; and (2) a one-step process with the same transgenes but without the chemical induction step and including an additional HRAS mutant transgene, HRASG12V. For both protocols, we inhibited TP53 expansion genes, which are hypothesized to confer unique cancer resistance in elephants. To confirm their reprogrammed state, we generated a functional genomics catalog of the modified cells with RNA-seq, ATAC-seq, and single-molecule direct RNA-sequencing. While these emiPSCs are transgene-dependent, we inactivated them to differentiate emiPSCs into all three germ layers via tri-lineage differentiation, embryoid body generation, and direct differentiation into putative cell types from all three layers. These methods will open new frontiers for cellular models of non-model organisms, including for genetic rescue and conservation.
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