Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1221018 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR32297328_1.fastq.gz | Fastqsanger.gz | |||
| SRR32297328_2.fastq.gz | Fastqsanger.gz | |||
| SRR32297329_1.fastq.gz | Fastqsanger.gz | |||
| SRR32297329_2.fastq.gz | Fastqsanger.gz | |||
| SRR32297330_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 18 |
Similar Datasets
Project description:Rabies is an ancient infectious disease but still lacking efficient therapeutic approach despite of vaccine. In this study, we have identified a novel cytoplasmic lncRNA, namely rabies virus related lncRNA 1(RVRL1), whose expression in neuronal cells is up-regulated upon the infection of the causative agent of rabies, the neurotropic virus rabies virus (RABV). RVRL1 effectively inhibits RABV infection both in neuronal cells and in a mouse model. RVRL1 binds to EZH2 and disrupts the PRC2 complex, which is consistent with the inverse relationship between RVRL1 expression and the cellular H3K27me3 level. RVRL1 expression positively regulates the expression of PCP4L1 encoding a 10 kD peptide, which is shown to inhibit RABV replication. These findings highlight a novel mechanism for lncRNAs to upregulate the expression of antiviral genes, and define two potential anti-rabies reagents including an antiviral lncRNA and an antiviral peptide.
2020-09-08 | GSE107285 | GEO
Project description:Street-strain rabies virus primarily replicates in central nervous system without inducing significant immune response or structural damages on neurons, but the manifested symptoms of rabies indicate inherent neuronal dysfunctions in CNS. To understand the underlying state of rabies virus-infected neurons and find probable mechanisms for the neuronal dysfunction, we performed RNA-Seq at multiple time-points. This dataset provides RNA-Seq results of wild-type and mutant rabies virus-infected neuron transcriptome, with clear differential expressions between conditions. Through comparative analysis of different time-points, we have found that the matrix protein of rabies virus plays an important role in early suppression of host gene expression and maintaining control over immune response and other processes. The signaling pathways previously known to interact with rabies virus were confirmed to be modulated in this dataset, and contribute to neuronal function-associated processes. We have verified the regulation of gene expressions that could impact neuronal functions collectively, and demonstrated in calcium imaging that indeed the oscillation of calcium trace in neurons are influenced by rabies virus infection.
2021-11-04 | GSE178583 | GEO
Project description:Rabies is an ancient infectious disease but still lacking efficient therapeutic approach despite of vaccine. In this study, we have identified a novel cytoplasmic lncRNA, namely rabies virus related lncRNA 1(RVRL1), whose expression in neuronal cells is up-regulated upon the infection of the causative agent of rabies, the neurotropic virus rabies virus (RABV). RVRL1 effectively inhibits RABV infection both in neuronal cells and in a mouse model. RVRL1 binds to EZH2 and disrupts the PRC2 complex, which is consistent with the inverse relationship between RVRL1 expression and the cellular H3K27me3 level. RVRL1 expression positively regulates the expression of PCP4L1 encoding a 10 kD peptide, which is shown to inhibit RABV replication. These findings highlight a novel mechanism for lncRNAs to upregulate the expression of antiviral genes, and define two potential anti-rabies reagents including an antiviral lncRNA and an antiviral peptide.
2020-09-08 | GSE107277 | GEO
Project description:Rabies is a fatal zoonotic disease posing a threat to the public health globally. Rabies virus (RABV) is excreted in the saliva of infected animals, and is primarily transmitted through bite contact. Salivary glands play an important role for virus propagation. However, the significance of salivary glands is less studied in RABV pathogenic mechanisms. To identify functionally important genes in the salivary glands, we employed RNA sequencing (RNA-seq) to establish and analyze mRNA expression profiles in parotid tissue infected with two RABV strains, CVS-11 and PB4. We map the transcriptome changes in response to RABV infection in parotid tissue for the first time. This work provides new clues to the study of RABV-affected salivary gland function and RABV transmission mechanisms in parotid tissue. And the salivary gland-enriched transcripts could be potential targets of interest for rabies disease control.
2023-11-26 | GSE248365 | GEO
Project description:<p>Pseudorabies virus infection causes intestinal flora disorder in animals, leading to diarrhea. To explore the effect of inactivated pseudorabies vaccine immunization on pseudorabies virus infection, this study sequenced the serum metabolome and intestinal microbiota of Hu sheep, analyzed their differential expression and conducted a combined analysis. The results showed that all the Hu sheep in the vaccine immunization group survived, while those in the non-immunization group died. The expressions of quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate, L-methionine, L-glutamic acid, thymine, adenine and 20-HETE were upregulated in the serum of the vaccine immunization group (P<0.05). The expressions of amifostine, hemialdehyde succinate and trehalose were down-regulated (P<0.05). The microbiota structure and abundance in the vaccine immunization group changed significantly, and the beneficial bacteria Christensenellaceae_R-7_group, Bifidobacterium, Solibacillus, and Rikenellaceae_RC9_gut_group increased. The results of the combined omics analysis showed that the changes of metabolites such as quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate and adenine were positively correlated with the abundance changes of Rikenellaceae_RC9_gut_group (P<0.05); L-methionine and 20-HETE were positively correlated with adenine and Solibacillus (P<0.05); Succinate semialdehyde was significantly negatively correlated with Rikenellaceae_RC9_gut_group. The above results indicate that after inactivated pseudorabies vaccine immunization of Hu sheep, it affects metabolites (quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate adenine, L-methionine and 20-HETE) by maintaining beneficial intestinal bacteria (Rikenella aceae_RC9_group and Solibacillus). Metabolites jointly regulate the generation of inflammatory mediators, influence the function of immune cells and participate in the regulation of oxidative stress, thereby exerting an immune protective effect. This study provides an important theoretical basis for a deeper understanding of the immune protection mechanism of inactivated pseudorabies vaccine.</p>
2025-09-03 | MTBLS12933 | MetaboLights
Project description:<p>Pseudorabies virus infection causes intestinal flora disorder in animals, leading to diarrhea. To explore the effect of inactivated pseudorabies vaccine immunization on pseudorabies virus infection, this study sequenced the serum metabolome and intestinal microbiota of Hu sheep, analyzed their differential expression and conducted a combined analysis. The results showed that all the Hu sheep in the vaccine immunization group survived, while those in the non-immunization group died. The expressions of quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate, L-methionine, L-glutamic acid, thymine, adenine and 20-HETE were upregulated in the serum of the vaccine immunization group (P<0.05). The expressions of amifostine, hemialdehyde succinate and trehalose were down-regulated (P<0.05). The microbiota structure and abundance in the vaccine immunization group changed significantly, and the beneficial bacteria Christensenellaceae_R-7_group, Bifidobacterium, Solibacillus, and Rikenellaceae_RC9_gut_group increased. The results of the combined omics analysis showed that the changes of metabolites such as quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate and adenine were positively correlated with the abundance changes of Rikenellaceae_RC9_gut_group (P<0.05); L-methionine and 20-HETE were positively correlated with adenine and Solibacillus (P<0.05); Succinate semialdehyde was significantly negatively correlated with Rikenellaceae_RC9_gut_group. The above results indicate that after inactivated pseudorabies vaccine immunization of Hu sheep, it affects metabolites (quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate adenine, L-methionine and 20-HETE) by maintaining beneficial intestinal bacteria (Rikenella aceae_RC9_group and Solibacillus). Metabolites jointly regulate the generation of inflammatory mediators, influence the function of immune cells and participate in the regulation of oxidative stress, thereby exerting an immune protective effect. This study provides an important theoretical basis for a deeper understanding of the immune protection mechanism of inactivated pseudorabies vaccine.</p>
2025-09-04 | MTBLS12936 | MetaboLights
Project description:Cortical circuit tracing using modified rabies virus can identify input neurons making direct monosynaptic connections onto neurons of interest. However, challenges remain in our ability to establish the cell type identity of rabies-labeled input neurons. While transcriptomics may offer an avenue to characterize inputs, the extent of rabies-induced transcriptional changes in distinct neuronal cell types remains unclear and whether these changes preclude characterization of rabies-infected neurons according to established transcriptomic cell types is unknown. We used single-nucleus RNA sequencing to survey the gene expression profiles of rabies-infected neurons and assessed their correspondence with established transcriptomic cell types. We demonstrated that when using transcriptome-wide RNA profiles, rabies-infected cortical neurons can be transcriptomically characterized despite global and cell-type-specific rabies-induced transcriptional changes. Notably, we found differential modulation of neuronal marker gene expression, suggesting that caution should be taken when attempting to characterize rabies-infected cells with single genes or small gene sets.
2022-05-25 | GSE196771 | GEO
Project description:This experiment is part of the project that primarily aims to utilize 3D hydrogel-based hiPSC-derived neuronal model to study rabies virus infection in the central nervous system. Having established the optimal 3D neuronal model, we then investigated the growth kinetics of two strains of rabies virus (TH and CVS-11) and comparatively analyzed the 2D and 3D culture models. We performed a gene expression analysis using NanoString to determine whether changes in gene expression could explain the differences in virus growth kinetics of two strains of rabies virus observed between the 2D and 3D neuronal culture models. Gene expression analysis of the neuropathological pathway observed during rabies virus infection demonstrated a vast number of differentially expressed genes in the 3D model as compared to the 2D model.
2023-08-23 | GSE231664 | GEO
Project description:This study investigates transcriptomic alterations upon rabies virus CVS strain infection in wild-type and RGS4-knockout mouse Neuro-2A(N2A) neuroblastoma cells. RGS4 is a potential regulator involved in neuronal biological processes, and its functional role during rabies virus(RABV CVS strain) infection remains poorly characterized. Total RNA-seq was applied to profile global gene expression of wild-type N2A and RGS4-knockout N2A cells under RABV-CVS infected conditions. The primary objective is to identify differentially expressed genes and dysregulated signaling pathways triggered by RGS4 deficiency during CVS rabies virus infection, to reveal host molecular networks modulated by RGS4 upon lyssavirus infection in neuronal cells. This RNA-seq dataset provides transcriptomic resources for dissecting the function of RGS4 in host rabies virus interaction.
2026-09-13 | GSE345814 | GEO
Project description:Rabies virus (RABV) infection led to alteration of microRNA expression in NA and microglia. A sixteen-chip study was performed using total RNA isolated from NA and microglia infected with rabies virus CVS-11 (Challenge virus standard) or mock infected as a control. RNA was isolated from microglia at 12, 24, or 48 hpi and from NA at 24 hpi.
2014-10-10 | E-GEOD-35706 | biostudies-arrayexpress