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Triticum turgidum subsp. durum x Triticum dicoccoides

ABSTRACT: Cloning of two NLR genes at YRTD121 locus from wild emmer wheat Raw sequence reads

PROVIDER: PRJNA1221773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32290233_1.fastq.gz	Fastqsanger.gz
	SRR32290233_2.fastq.gz	Fastqsanger.gz
	SRR32290234_1.fastq.gz	Fastqsanger.gz
	SRR32290234_2.fastq.gz	Fastqsanger.gz
	SRR32290235_1.fastq.gz	Fastqsanger.gz

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Project description:Cloning of two NLR genes at YRTD121 locus from wild emmer wheat

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TdERF1, an ethylene response factor associated with dehydration responses in durum wheat (Triticum turgidum L. subsp. durum).

Project description:Water deficit and increasing salinization reduce productivity of wheat, the leading crop for human diet. While the complete genome sequence of this crop has not been deciphered, a BAC library screening allowed the isolation of TdERF1, the first ethylene response factor gene from durum wheat. This gene is putatively involved in mediating salt stress tolerance and its characterization provides clues toward understanding the mechanisms underlying the adaptation/tolerance of durum wheat to suboptimal growth conditions. TdERF1 expression is differentially induced by high salt treatment in 2 durum wheat varieties, the salt-tolerant Grecale (GR) and the salt-sensitive Om Rabiaa (OR). To further extend these findings, we show here that the expression of this ERF is correlated with physiological parameters, such as the accumulation of osmo-regulators and membrane integrity, that discriminate between the 2 contrasted wheat genotypes. The data confirm that GR and OR are 2 contrasted wheat genotypes with regard to salt-stress and show that TdERF1 is also induced by water stress with an expression pattern clearly discriminating between the 2 genotypes. These findings suggest that TdERF1 might be involved in responses to salt and water stress providing a potential genetic marker discriminating between tolerant and sensitive wheat varieties.

| S-EPMC4883918 | biostudies-other

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