Project description:Small RNAs play important roles in photoperiodism, but a comprehensive characterization of miRNAs and the more recently recognized tRFs within this context is lacking. Herein, we characterize the patterns of miRNAs and tRFs in Arabidopsis by performing time-course sRNA-sequencing across three photoperiods. By comparing with our previous mRNA-sequencing time-courses (GSE221846), we identified 14 negatively-correlated miRNA-mRNA pairs between the two co-sequenced datasets that suggest photoperiodic sRNA regulations. Furthermore, we identified 20 patterns of photoperiodic tRFs. These patterns are linked to the tRNA isotypes and positions they derive from, suggesting that tRFs are subjected to photoperiodic regulations.
Project description:af51_atm - atm time course - which Arabidopsis thaliana genes are induced by ionising radiations? Comparison of SADE transcriptome data (Af1999083) and micro-arrays transcriptome data. - which Arabidopsis thaliana genes are induced by ionising radiations ? time course: 0.75h, 1.5h, 3h, 5h dose: Gamma rays irradiated vs non irradiated 4-days old seedlings Keywords: time course
Project description:Arabidopsis thaliana wildtype (Col-0) was compared with the ntrc mutant under different photoperiods (short day: 8h/16h; long day 16h/8h light/dark) and different ages (10d and 28/21d).
Project description:Photoperiod is a circannual signal measured by biological systems to align growth and reproduction with the seasons. To understand the effect of photoperiod of gene expression in Arabidopsis thaliana in the absence of exogenous sugar under constant light intensity, we performed time course mRNA-seq analysis on 13-day old seedlings across three photoperiods with triplicates to identify photoperiod-regulated genes.