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Rumen microbial 16s sequencing by in vitro fermentation test

ABSTRACT: Rumen microbial 16s sequencing by in vitro fermentation test Raw sequence reads

PROVIDER: PRJNA1221970 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32304526_1.fastq.gz	Fastqsanger.gz
	SRR32304526_2.fastq.gz	Fastqsanger.gz
	SRR32304527_1.fastq.gz	Fastqsanger.gz
	SRR32304527_2.fastq.gz	Fastqsanger.gz
	SRR32304528_1.fastq.gz	Fastqsanger.gz

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Project description:Diseases caused by parasitic flatworms of rumen tissues (paramphistomosis) are a significant threat to global food security as a cause of morbidity and mortality in ruminant livestock in subtropical and tropical climates. Calicophoron daubneyi is currently the only paramphistome species commonly infecting livestock species in temperate European climates. However, recorded incidences of C. daubneyi infection in European livestock have been increasing over the last decade. Whilst clinical paramphistomosis caused by adult worms is currently uncommon in Europe, fatalities have been reported in association with severe haemorrhagic enteritis resulting from the migration of immature paramphistomes. Large numbers of mature adults can reside in the rumen, yet to date, the impact on rumen fermentation, and consequently in productivity and economic management of infected livestock have not been resolved. Limited public available nucleotide and protein sequences for C. daubneyi underpin this lack of biological and economic understanding. Here we present for the first time a de novo assembled transcriptome, with functional annotations, for adult C. daubneyi, which provides a reference database for protein and nucleotide sequence identification to facilitate fundamental biology, anthelmintic, vaccine and diagnostics discoveries. This dataset identifies a number of genes potentially unique to C. daubneyi and, by comparison to an existing transcriptome for the related Paramphistomum cervi, identifies novel genes which may be unique to the paramphistome group of platyhelminthes. Additionally, we present the first coverage of the excretory/secretory and soluble somatic proteome profiles for adult C. daubneyi and identify the release of extracellular vesicles from adult C. daubneyi parasites during in vitro, ex-host culture. Finally, we have performed the first analysis of rumen fluke impacting upon rumen fermentation parameters using an in vitro gas production study resulting in a significant increase in propionate production.

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Rumen microbial 16s sequencing by in vitro fermentation test

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