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Forskolin induced pluripotency of caudal fin fibroblasts of Topmouth culter.

ABSTRACT: Forskolin induced pluripotency of caudal fin fibroblasts of Topmouth culter.

PROVIDER: PRJNA1222417 | ENA |

REPOSITORIES: ENA

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Forskolin induced pluripotency of caudal fin fibroblasts of Topmouth culter.

Project description:As the passage number increases, the spontaneous aggregation phenomenon of TCCF2022 cells gradually weakens and eventually disappears, and occasional instances of post-passage cells being unable to proliferate are observed. This suggests that the characteristics of the cells may have changed during the culture process.We found that when individual factors were used to induce TCCF2022, on the third day after treatment with 5μM Forskolin, TCCF2022 cells regained the ability to form cell aggregates, and alkaline phosphatase detection was also positive. Therefore, we need to determine the possible mechanism of this state recovery through RNA-seq.

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Single-cell RNA sequencing of the cut and uncut caudal fin of zebrafish larvae

Project description:Purpose: The goal of this study was to establish the first detailed cell atlas of the regenerating caudal fin of zebrafish larvae. Intact and regenerating caudal fin were used for single-cell RNA-sequencing with the aim to provide the first integrated model of epimorphic regeneration in zebrafish larvae and demonstrate the diversity of the cells required for blastema formation. Methods: 150 of regenerating caudal fin (cut) and intact caudal fine (uncut) samples were dissociated and loaded into the 10x Genomics Chromium Platform, and sequenced using Illumina NovaSeq 6000. Conclusion: Our study constitutes a resource of the gene expression profile in intact and regenerating caudal fin of zebrafish larvae. We report the application of single-molecule-based sequencing technology for high-throughput profiling of both intact (uncut) and regenerating caudal fin samples (cut) at 24hpA. We confirmed the presence of macrophage subsets, previously described by our group to govern zebrafish fin regeneration, and identified a novel blastemal cell population.

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