Single-cell RNA seq of developing and smoc1/2 ko mouse incisor
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ABSTRACT:
PROVIDER: PRJNA1222787 | ENA |
REPOSITORIES: ENA
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Project description:We used single-cell sequencing to understand the development of incisor tissues from mice. We utilized the single-cell sequencing platform from SeekGene Biotechnology to study the heterogeneity of mesenchyme in incisors at both embryonic and adult stages of mice, and discovered that Smoc2 is a marker gene for mesenchymal stem cells in mouse incisors. We employed the 10X platform to sequence the incisor tissues of smoc1/2 knockout (ko) and control mice during the embryonic stage to investigate the molecular mechanisms underlying the developmental abnormalities in smoc1/2 ko mice.Our study provides a molecular mechanism explaining how stem cells form during development.
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Project description:Purpose: Analysis the heterogeneity of the MSC population in the adult mouse incisor, using single cell RNA-sequence. Results: About 9,318 cells (~63,000 reads per cells) were successfully barcoded and their transcriptomes sequenced.
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Project description:miRNA expression was compared in 3 distinct regions of the adult mouse incisor: the labial cervical loop, which houses ameloblast stem cells; the lingulal cervical loop, which houses stem cells but not ameloblast stem cells; and ameloblasts. Differentially expressed miRNAs from these regions are likely involved in the renewal and differentiation of stem cells. miRNA expression in the labial and lingual cervical loops, and ameloblasts were compared from 5 specimens.
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