Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1226917 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We cultivated two halo-alkaliphilic cyanobacteria consortia in chemostats at pH 10.2-11.4. One consortium was dominated by Ca. Sodalinema alkaliphilum, the other by a species of Nodosilinea. These two cyanobacteria dominate natural communities in Canadian and Asian alkaline soda lakes. We show that increasing the pH decreased biomass yield. This decrease was caused, in part, by a dramatic increase in carbon transfer to heterotrophs. At pH 11.4, cyanobacterial growth became limited by bicarbonate uptake, which was mainly ATP-dependent. In parallel, the higher the pH, the more sensitive cyanobacteria became to light, resulting in photoinhibition and upregulation of DNA repair systems.
2024-10-17 | PXD052563 | Pride
Project description:Despite cultivation of many representatives of rich bacterial communities inhabiting alkaline soda lakes, members of the bacterial phylum Verrucomicrobiota have so far been detected only by molecular techniques. Here, we used alginate as a selective substrate to enrich and isolate two strains of haloalkaliphilic Verrucomicrobiota. The isolates share identical 16S rRNA gene sequences and represent a new genus, and, together with metagenome assembled genomes, a new family within Opitutales. Cells of strains AB-alg1T (from soda lakes) and AB-alg4 (from soda soils) are small motile cocci forming submerged colonies in soft alginate agar. They are saccharolytic heterotrophs growing aerobically on polysaccharides (alginate, starch and inulin) and sugars (glucose, fructose, mannose, sucrose, melezitose, maltose and cellobiose). They also grow anaerobically by fermentation of alginate and D-mannose and by coupling incomplete denitrification to oxidation of alginate. Both isolates are obligately alkaliphilic and moderately salt-tolerant. The dominant membrane phospholipids include phosphatidylcholine and cardiolipin. The genome of AB-alg1T features polysaccharide lyases of the PL6, 7, 15, 17, 38, and 39 families for depolymerization of alginate. On the basis of phenotypic and distinct phylogeny, we propose classification of strains AB-alg1T (JCM 35393T=UQM 41574T) and AB-alg4 as Verruconatronum alginilyticum gen. nov., sp. nov. within a new family Verruconatronumaceae.
2026-04-17 | PXD071159 | Pride
Project description:Giant viruses infecting amoebae are notable for their substantial particle sizes and expansive genomes. In this study, we collected water samples from 44 distinct locations along a river within Brazil's Pantanal biome. These samples were processed and subsequently inoculated into Vermamoeba vermiformis cultures. Observation of cytopathic effects led to the isolation of a novel virus, designated Naiavirus, distinguished by its unprecedented virion morphology and genomic organization. To elucidate the protein composition of Naiavirus particles, large-scale infections were conducted using 20 T175 flasks, each containing 10 million amoebae. Post-infection, viral particles were purified through two successive sucrose gradient centrifugations to achieve high purity. Proteomic analysis of these purified particles identified 254 associated viral proteins, a count exceeding those reported for all known giant viruses except for pandoraviruses. This dataset encompasses the raw mass spectrometry data, identification files, and associated metadata, offering valuable insights into the structural proteome of this unique giant virus.
2025-09-29 | PXD063493 | Pride
Project description:The central part of Brazil, consisting mostly of the Cerrado Biome, is considered to be the new frontier for increasing Brazilian wheat production. However, rainfed wheat production in that area must cope with drought stress. In order to better understand the drought response, we analyzed the mRNA profiling under drought in roots and leaves of the cultivar MGS1 Aliança (a well-adapted cultivar to the Cerrado). We identified 4,422 candidate genes in roots and leaves.
2016-11-29 | GSE81833 | GEO
Project description:In this study we used metaproteomics to discern the metabolism and physiology of the microorganisms occurring in the phototrophic mats of four soda lakes in the interior of British Columbia, Canada. Binned and assembled metagenomes were used as the database for protein identification.
2019-07-29 | PXD011230 | Pride