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Dictyotopsis propagulifera

ABSTRACT: Dictyotopsis propagulifera and related species organelle genome assembly

PROVIDER: PRJNA1227714 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32583815.fastq.gz	Fastqsanger.gz
	SRR32582074.fastq.gz	Fastqsanger.gz
	SRR32613482_1.fastq.gz	Fastqsanger.gz
	SRR32613482_2.fastq.gz	Fastqsanger.gz

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Comparative RNA-Seq analysis reveals potentially resistance-related genes in response to Bacterial canker of tomato

Project description:Tomato is one of the most important crops for human consumption. Unfortunately, its production is affected by diseases caused by pathogens such as the actynomicete Clavibacter michiganensis subsp. michiganens (Cmm). This pathogen is the causal agent of the bacterial canker of tomato, considered one of the most devastating tomato diseases. To date, there are not resistant varieties of commercial tomato against Cmm. However, there are wild tomato species resistant to Cmm. Using massive sequencing, we obtained the transcriptomes of the wild tomato species Solanum arcanum LA2157 and the commercial tomato Solanum lycopersicum cv. Ailsa Craig at 8 and 24 hours after Cmm challenge. We identified potential tomato tolerance-related genes by three approaches: mapping the reads to S. lycopersicum reference genome SL3.0, performing a semi de novo transcriptome assembly and a de novo transcriptome assembly. Some functional groups such as oxylipin biosynthetic process response to wounding, response to cytokinin among others, were enriched in both tomato species, suggesting a similar response, however, genes that encode proteins such as the Polyphenol oxidase E, Ankyrin and Leucine Rich Repeat receptors were overexpressed mainly in the wild tomato species, suggesting a possible role in the defense response. Here, we uncovered new candidate genes potentially related to bacterial canker tomato defense.

2020-06-13 | GSE152330 | GEO

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