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Hyalomma anatolicum

ABSTRACT: Hyalomma anatolicum salivary gland transcriptome from infected and uninfected Theileria annulata

PROVIDER: PRJNA1227817 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32628700_1.fastq.gz	Fastqsanger.gz
	SRR32628700_2.fastq.gz	Fastqsanger.gz
	SRR32628701_1.fastq.gz	Fastqsanger.gz
	SRR32628701_2.fastq.gz	Fastqsanger.gz
	SRR32628702_1.fastq.gz	Fastqsanger.gz

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Modulation of activation-associated host cell gene expression by the apicomplexan parasite Theileria annulata

2012-10-01 | GSE36428 | GEO

miRNA profiling of extracellular vesicles from Theileria annulata infected B cells (TBL20) and their uninfected control counterparts (BL20)

Project description:We report significant changes in six bovine miRNA in vesicles from Theileria annulata infected cells

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Modulation of activation-associated host cell gene expression by the apicomplexan parasite Theileria annulata

Project description:The experiment investigates bovine gene expression in response to LPS in uninfected and Theileria annulata-infected cell cultures A subset of genes are identified which are activated in response to LPS stimulation with further modulation due to parasite infection. Six experimental conditions with three replicates per condition. Total RNA prepared from cell cultures. BL20 (uninfected bovine lymphosarcoma cell line), BL20 4 hours post-LPS stimulation, BL20 18 hours post-LPS, TBL (T. annulata infected bovine cell line), TBL 4 hours post-LPS, TBL 18 hours post-LPS. Each hydridisation represents bovine and parasite gene expression on a single channel and 2 technical replicates of each probeset are represented on the chip.

2012-10-01 | E-GEOD-36428 | biostudies-arrayexpress

Project description:De Novo Assembly and Differential Transcriptomic Analysis of Hyalomma anatolicum Salivary Glands in Response to Theileria Annulata

| PRJNA1011479 | ENA

RNA Seq Profiling of Murine Salivary Glands in the Presence or Absence of Active MCMV Infection

Project description:Recent work indicates that salivary glands are able to constitutively recruit CD8+ T cells and retain them as tissue resident memory T cells (TRM), independently of local infection, inflammation or antigen. To understand the mechanisms supporting T cell recruitment to the salivary gland, we compared T cell migration to the salivary gland in mice infected or not with murine cytomegalovirus (MCMV), a herpesvirus that infects the salivary gland and promotes the accumulation of salivary gland TRM. We found that acute MCMV infection increased rapid T cell recruitment to the salivary gland, but that equal numbers of activated CD8+ 44 T cells eventually accumulated in both infected and uninfected glands. T cell recruitment to uninfected salivary glands depended on chemokines and the integrin α4. Several chemokines were expressed in the salivary glands of both infected and uninfected mice and many of these could promote the migration of MCMV-specific T cells in vitro. MCMV infection increased expression of chemokines that interact with the receptors CXCR3 and CCR5, but neither receptor was needed for T cell recruitment to the salivary gland during MCMV infection. Unexpectedly however, the chemokine receptor CXCR3 was critical for T cell accumulation in uninfected salivary glands. Together, these data suggest that CXCR3 and the integrin α4 mediate T cell recruitment to uninfected salivary glands, but that redundant mechanisms mediate T cell recruitment after MCMV infection.

2018-01-15 | GSE107338 | GEO

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