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ABSTRACT: microRNA influences the proliferation of Nosema ceranae in Apis ceranae

PROVIDER: PRJNA1228290 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32488577_1.fastq.gz	Fastqsanger.gz
	SRR32488577_2.fastq.gz	Fastqsanger.gz
	SRR32488578_1.fastq.gz	Fastqsanger.gz
	SRR32488578_2.fastq.gz	Fastqsanger.gz
	SRR32488579_1.fastq.gz	Fastqsanger.gz

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Proteomics of anatomical sections of the gut of Nosema-infected Western honey bee (Apis mellifera) reveals different early responses to Nosema spp. isolates

Project description:We aim to evaluate the effects of four Nosema spores’ isolates, (i) and (ii) N. ceranae isolated from A. mellifera hosts from two different geographical origins, (iii) N. ceranae from A. cerana host and (iv) N. apis from A. mellifera, on the A. mellifera on gut proteomics at the early stage of infection. To dissect the molecular mechanism responsible of the susceptibility of A. mellifera to Nosema, we investigated by high-resolution proteomics (LC-ESI-MS/MS) and differential label-free quantification of proteins (LFQ) the molecular cross-talk existing between different species and isolates of N. apis and N. ceranae, and the targetted gut tissue of A. mellifera. To reach the objectives of this study, we performed a bottom-up proteomic analysis on the different anatomical sections of the gut tissue (esophagus, crop, midgut, ileum and rectum) at an early stage of the exposition to Nosema spores (4 days). Then, we focused on the midgut, the region targeted by Nosema sposres for germination and, as we found out, the second region with the highest load of Nosema proteins, after the rectum, to perform differential quantitative proteomic analyses and acquire series of up- and down-regulated proteins. We discussed the different pathways observed to be impacted by different Nosema species and isolates with a main focus on the deregulated metabolic and response to stimuli processes.

2020-11-25 | PXD021848 | Pride

Trancriptome analysis of honey bees (Apis mellifera) infected with Nosema ceranae

Project description:The microsporidia Nosema ceranae are intracellular parasites that proliferate in the midgut epithelial cells of honey bees (Apis mellifera). To analyze the pathological effects of those microsporidia, we orally infected honey bee workers 7 days after their emergence. Bees were flash frozen 15 days after the infection. Then, the effects on the gut ventriculi were analyzed and compared to non-infected (control) bees. Comparisons of control vs Nosema ceranae bees

2011-08-31 | E-GEOD-25455 | biostudies-arrayexpress

Project description:microRNA regulates the proliferation of Nosema ceranae in Apis cerana

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Impact of Nosema apis and Nosema ceranae co-infection on worker fat body gene expression

Project description:Newly emerged adult workers (24 hours old) were infected with 25,000 Nosema apis spores and 25,000 Nosema ceranae spores in sucrose solution. Controls were fed sucrose. Workers were maintained in cages in an incubator and collected at 14 days post-infection. Fat bodies (eviscerated abdomens) were dissected and whole genome expression in this tissue was compared across treatments using microarrays.

2014-05-01 | E-MEXP-3889 | biostudies-arrayexpress

Trancriptome analysis of honey bees (Apis mellifera) infected with Nosema ceranae

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Project description:Nosema ceranae infection of Apis mellifera

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Project description:Apis mellifera and Nosema ceranae infection

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Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae

Project description:In this study we addressed whether the transcriptome profile in the honey bee brain is similar for two major parasites of honey bee, Varroa destructor and Nosema ceranae. Honey bees parasitized by these two parasites show accelerated behavioral maturation and deficiences in orientation and learning/memory that we hoped to characterized at the transcriptomic level. honey bee adults infested by Varroa destructor or Nosema ceranae compared to control bees, in duplicate

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Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae

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