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ABSTRACT: CDC Mycotic Diseases Branch Cadophora species sequencing

PROVIDER: PRJNA1228662 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR32499014_1.fastq.gz	Fastqsanger.gz
	SRR32499014_2.fastq.gz	Fastqsanger.gz
	SRR32499015_1.fastq.gz	Fastqsanger.gz
	SRR32499015_2.fastq.gz	Fastqsanger.gz
	SRR32499016_1.fastq.gz	Fastqsanger.gz

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Project description:Dendritic cells (DC) are professional antigen presenting cells that develop from hematopoietic stem cells in bone marrow by successive steps of lineage commitment and differentiation. Different DC subsets were identified based on phenotype, localisation and function: (i) classical DC (cDC) and plasmacytoid DC (pDC) are found in lymphoid organs and (ii) migratory tissue DC are spread throughout peripheral organs, including Langerhans cells, the cutaneous contingent of DC. We have developed a two-step culture system that recapitulates DC development in vitro (Felker et al., J. Immunol. 185, 5326-5335, 2010). In this system multipotent hematopoietic progenitors (MPP) progress into DC-restricted common DC progenitors (CDP) and further into the two major DC subsets cDC and pDC. We employed chromatin immunoprecipitation (ChIP) with deep sequencing (ChIP-seq) to determine the dynamics of H3K27ac occupancy in MPP, CMP, cDC and pDC. Histone modification H3K27ac and RNA-Seq in MPP, CDP, cDC and pDC

2016-08-03 | E-GEOD-73143 | biostudies-arrayexpress

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