Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Shared roles of immune and stromal cells in the pathogenesis of human bronchiolitis obliterans syndrome

ABSTRACT: Shared roles of immune and stromal cells in the pathogenesis of human bronchiolitis obliterans syndrome

PROVIDER: PRJNA1230197 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome changes in 2,3-butanedione induced bronchiolitis obliterans in rats

Project description:Bronchiolitis obliterans (BO) is a destructive fibrotic lung disease, which can be partly induced by 2,3-butanedione (also known as diacetyl, DA). However, the mechanism underlying the effects of DA on BO is not clear. In the present study, a bioinformatics analysis was performed using DA-treated or untreated lung tissues of rats.

2025-01-22 | GSE279398 | GEO

Shared roles of immune and stromal cells in the pathogenesis of human bronchiolitis obliterans syndrome.

Project description: Not available

| S-EPMC12128974 | biostudies-literature

Shared roles of immune and stromal cells in the pathogenesis of human bronchiolitis obliterans syndrome

Project description:We performed single cell RNA sequencing using the 10X genomics platform on explanted human lungs from 4 CLAD-BOS patients, 3 cGVHD-BOS patients, and 3 deceased controls to identify cell types, genes, and pathways enriched in BOS to better understand disease mechanisms

2025-04-10 | GSE290834 | GEO

Transcriptome changes in 2,3-butanedione induced bronchiolitis obliterans in rats

Project description:Transcriptome changes in 2,3-butanedione induced bronchiolitis obliterans in rats

| PRJNA1172638 | ENA

Plasma Biomarkers of Postinfectious Bronchiolitis Obliterans and Asthma in Children

Project description:Asthma and postinfectious bronchiolitis obliterans (PIBO) are chronic lung diseases characterized by recurrent episodes of wheezing. Mycoplasma, adenovirus, and respiratory syncytial virus infections can trigger both asthma and PIBO. These two diseases have common etiologic mechanisms that cause airway epithelial injury. They are often difficult to differentiate clinically in preschool children because both are exacerbated by viral infections and respond similarly to steroids and β2 agonists. PIBO, which is occasionally observed in children, is diagnosed through characteristic findings of air trapping on computed tomography or in biopsy samples of lung tissue. However, researchers have not clearly identified the specific blood markers that can distinguish these diseases or the differences in the mechanisms of development. We performed proteomic analysis of plasma to identify specific biomarkers that can be helpful in differentiating asthma from PIBO. This study discovered plasma biomarker candidates by measuring plasma proteome sequential window acquisition of all theoretical mass spectra (SWATH-MS) and included 30 healthy children, 18 with asthma and 15 with PIBO. was used to measure proteins in plasma samples. We identified and quantified 354 proteins across all 63 samples in the SWATH-MS analysis.

2022-12-01 | PXD034996 | Pride

Spatial Transcriptomics Analysis of Macrophage-to-Myofibroblast Transition in Bronchiolitis Obliterans Following Hematopoietic Stem Cell Transplantation

Project description:Bronchiolitis obliterans (BO) is a severe, fibrotic manifestation of chronic graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation (HSCT). Macrophage-derived TGF-β is linked to GVHD-related fibrosis; however, its role in BO remains unclear. Given emerging evidence of macrophage-to-myofibroblast transition (MMT) in fibrosis, this study aimed to investigate whether MMT contributes to BO development. We analyzed lung samples from three HSCT recipients with BO who underwent lung transplantation, with tissues from two patients with lung cancer as controls. Spatial transcriptomics was performed to profile gene expression in foamy macrophages across anatomical regions and histological stages, compared to control peribronchiolar regions.

2026-01-22 | GSE316672 | GEO

A window into disease: Protein signatures of remodeled airways in transplanted lungs with Bronchiolitis Obliterans Syndrome

Project description:Background: Bronchiolitis obliterans syndrome (BOS), the most common form of chronic lung allograft dysfunction (CLAD), is the major limitation to long-term survival after lung transplantation. The patho-anatomical correlate is progressive, fibrotic occlusion of the small airways, so-called obliterative bronchiolitis (OB) –lesions, ultimately leading to organ failure. The molecular composition of these lesions is unknown. Methods: By combining laser-capture microdissection (LCM) and optimized sample preparation protocols for mass spectrometry (MS) we analyzed end-stage OB-lesions identified in explanted lungs from four end-stage BOS patients. Immunohistochemistry and immunofluorescence were used to follow selected proteins of interest on the tissue level. Results: We established protein signatures for in total 15 OB-lesions. A set of 12 proteins identified in all lesions included both distinct structural proteins (collagen type IV and VI) and cellular components (actins, vimentin, tryptase). Each respective lesion exhibited a unique composition of proteins (on average n=66), thereby mirroring the histomorphological variation of the lesions. Conclusions: Even though being distinct and focal pathologic events, OB-lesions showed considerable variations in their protein content, most likely reflecting different disease pathways. Combining spatial information and advanced protein identification, this study provides molecular and morphological insights essential for a better understanding of the development of chronic rejection after lung transplantation.

2021-05-19 | PXD014171 | Pride

The mononuclear phagocyte system contributes to fibrosis in post-transplant Obliterans Bronchiolitis

Project description:The mononuclear phagocyte system contributes to fibrosis in post-transplant Obliterans Bronchiolitis

| PRJNA674367 | ENA

The mononuclear phagocyte system contributes to fibrosis in post-transplant Obliterans Bronchiolitis

Project description:Bronchiolitis Obliterans Syndrome (BOS) is a fibrotic disease heavily responsible for high mortality rates after lung transplantation. Myofibroblasts are primary effectors of this fibrotic process, but their origin is still under debate. The purpose of this work was to identify the precursors of mesenchymal cells responsible for post-transplant airway fibro-obliteration. Lineage-tracing tools were used to track or deplete potential sources of myofibroblasts in the heterotopic tracheal transplantation model. Allografts were analysed by histology, confocal microscopy, flow cytometry or single-cell transcriptomic analysis. BOS explants were evaluated by histology and confocal microscopy. Myofibroblasts in the allografts were recipient-derived. Still, when recipient mice were treated with tacrolimus, we observed rare epithelial-to-mesenchymal transition phenomena and an increase in donor-derived myofibroblasts (P=0.0467), but the proportion of these cells remained low (7%). Hematopoietic cells, and specifically the mononuclear phagocyte system, gave rise to the majority of myofibroblasts found in occluded airways. Ablation of Cx3cR1+ cells decreased fibro-obliteration (P=0.0151) and myofibroblasts accumulation (P=0.0020). Single-cell RNA-sequencing unveiled similarities between myeloid-derived cells from allografts and both murine and human samples of lung fibrosis. Finally, myofibroblasts expressing the macrophage marker CD68 were increased in BOS explants when compared to controls (13% vs 2.3%, P=0.0007). Recipient-derived myeloid progenitors represent a clinically-relevant source of mesenchymal cells infiltrating the airways after allogeneic transplantation. Therefore, therapies targeting the mononuclear phagocyte system could improve long-term outcomes after lung transplantation.

2020-11-04 | GSE160760 | GEO

C5aR1 regulates T-follicular-helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans

Project description:C5aR1 regulates T-follicular-helper differentiation and chronic graft-versus-host disease bronchiolitis obliterans

| PRJNA498508 | ENA