Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites

ABSTRACT: FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites

PROVIDER: PRJNA1230219 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites

Project description:The first post-transcriptional step in mammalian mitochondrial gene expression, required for the synthesis of the 13 polypeptides encoded in mtDNA, is endonucleolytic cleavage of the primary polycistronic transcripts. Excision of the mtDNA-encoded tRNAs releases most mature RNAs; however, processing of three non-canonical mRNAs not flanked by tRNAs (CO1, CO3, CYB) requires FASTKD5. To investigate the molecular mechanism involved, we created knockout human cell lines to use as assay systems. The absence of FASTKD5 produced a severe OXPHOS assembly defect due to the inability to translate two unprocessed non-canonical mRNAs and predicted altered folding patterns specifically at the 5’-end of the CO1coding sequence. Structural features 13-15 nt upstream of the CO1 and CYB cleavage sites suggest FASTKD5 recognition mechanisms. Remarkably, a map of essential FASTKD5 amino acid residues revealed RNA substrate specificity; however, a key, putative active site residue was required for processing all three non-canonical pre-RNAs. Mutating this site resulted in a substantial increase in binding affinity with all client RNA substrates. A reconstituted in vitro system showed that wild-type, but not mutant, FASTKD5, was able to cleave client substrates correctly. These results establish FASTKD5 as the missing piece of biochemical machinery required to completely process the primary mitochondrial transcript.

2025-06-29 | GSE290841 | GEO

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs

Project description:U4 inhibits premature cleavage and polyadenylation of pre-mRNAs

| PRJNA1091625 | ENA

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [ChIP-seq]

Project description:To understand how U4 snRNP regulates premature cleavage and polyadenylation of pre-mRNAs at the transcriptome wide, we conducted RNAPII ChIP-seq analysis on control, U1 and U4-AMO treated samples.

2024-06-26 | GSE262384 | GEO

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA-seq]

Project description:To understand how U4 snRNP regulates premature cleavage and polyadenylation of pre-mRNAs at the transcriptome wide, we conducted mRNA-seq analysis on control, U1 and U4-AMO treated HeLa cells

2024-06-26 | GSE262380 | GEO

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA 3'-seq]

Project description:To understand how U4 snRNP regulates premature cleavage and polyadenylation of pre-mRNAs at the transcriptome wide, we conducted 3'-seq analysis on control, U1 and U4-AMO treated samples using the Lexogen mRNA 3'-seq kit (Cat. 016.24), which enables accurate quantification of global PAS usage.

2024-06-26 | GSE262383 | GEO

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [ChIP-seq]

Project description:U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [ChIP-seq]

| PRJNA1091630 | ENA

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA-seq]

Project description:U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA-seq]

| PRJNA1091620 | ENA

U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA 3'-seq]

Project description:U4 inhibits premature cleavage and polyadenylation of pre-mRNAs [mRNA 3'-seq]

| PRJNA1091628 | ENA

U6 snRNP inhibits the premature cleavage and polyadenylation of pre-mRNAs (ChIP-Seq)

Project description:U6 snRNP inhibits the premature cleavage and polyadenylation of pre-mRNAs (ChIP-Seq)

| PRJNA1053389 | ENA

U6 snRNP inhibits the premature cleavage and polyadenylation of pre-mRNAs (RNA-Seq)

Project description:U6 snRNP inhibits the premature cleavage and polyadenylation of pre-mRNAs (RNA-Seq)

| PRJNA1053391 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data