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Electrostatic properties of disordered regions control transcription factor search and pioneer activity [ChIP-Seq]

ABSTRACT: Electrostatic properties of disordered regions control transcription factor search and pioneer activity [ChIP-Seq]

PROVIDER: PRJNA1230564 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR32544784_1.fastq.gz	Fastqsanger.gz
	SRR32544784_2.fastq.gz	Fastqsanger.gz
	SRR32544785_1.fastq.gz	Fastqsanger.gz
	SRR32544785_2.fastq.gz	Fastqsanger.gz

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Project description:Transcription factors (TFs) recognize specific target sites within cis-regulatory regions to control gene expression. Prokaryotic TFs combine 1D and 3D diffusion mechanisms to efficiently locate their binding sites. In contrast, the determinants of target search efficiency of eukaryotic TFs within the chromatinized genome are poorly characterized. Here we combine in vivo and in vitro single molecule imaging to dissect the role of charged disordered regions of the mammalian Sox2 and Sox17 TFs in their ability to search for their binding sites. We demonstrate that the DBD flanking region of Sox2 (DFRSox2) dramatically enhances Sox2 search efficiency as compared to the negatively-charged DFRSox17 through distinct mechanisms on naked versus nucleosomal DNA. Enhanced search on naked DNA is primarily mediated by an increase in target recognition rate during 1D sliding. In contrast, target site recognition within nucleosomal DNA is mainly enhanced by increased nonspecific interactions with nucleosomes, facilitating binding to closed chromatin and reinforcing pioneering activity. These findings provide critical insights into the biophysical mechanisms governing TF target search beyond the DBD, and demonstrate how charged disordered regions modulate TF target search on the chromatinized genome.

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Intrinsically disordered domain of transcription factor TCF-1 is required for T cell developmental fidelity

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