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ABSTRACT: Identification of novel candidate genes for early heading on chromosome 5B in wheat mutant jg1489,RNA-Seq

PROVIDER: PRJNA1230783 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32549705_1.fastq.gz	Fastqsanger.gz
	SRR32549705_2.fastq.gz	Fastqsanger.gz
	SRR32549706_1.fastq.gz	Fastqsanger.gz
	SRR32549706_2.fastq.gz	Fastqsanger.gz
	SRR32549707_1.fastq.gz	Fastqsanger.gz

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Project description:Identification of novel candidate genes for early heading on chromosome 5B in wheat mutant jg1489

| PRJNA1230349 | ENA

Fumarylacetoacetate hydrolase targeted by a Fusarium graminearum effector positively regulates wheat FHB resistance and yield and promotes heading

Project description:Fusarium head blight (FHB), caused by Fusarium graminearum is a devastating disease that affects global wheat production. F. graminearum encodes many effector proteins; however, its virulence mechanisms are poorly understood. In this study, we identified a secretory effector candidate protein (FgEC10) that is essential for the virulence of F. graminearum and suppresses the basal immune response. FgEC10 interacted strongly with wheat fumarylacetoacetate hydrolase (TaFAH) and accelerated its degradation via the 26S proteasome pathway. In addition, we showed that TaFAH interacted with TaPSMD, an important component of the 26S proteasome and that FgEC10 enhanced the interaction between TaFAH and TaPSMD. RNA silencing or overexpression of TaFAH in wheat plants showed that TaFAH positively regulated wheat FHB resistance, heading period, and grain yield. Transcriptomic analysis revealed that TaFAH promoted the expression of genes associated with disease resistance and the heading period. Metabolomic analysis revealed that overexpression of TaFAH increased the levels of several amino acids that inhibited F. graminearum growth and enhanced wheat resistance to FHB. Collectively, our study reveals a novel pathogenic mechanism and provides a valuable genetic resource for improving FHB resistance and grain yield in wheat.

2024-08-15 | GSE254427 | GEO

Project description:early heading date_BSA_Ci846 mutant library

| PRJNA1155257 | ENA

Transcription profiling by array of wheat and barley to anchor physical and genetic maps in wheat using a barley array

Project description:Background Because of its size, allohexaploid nature and high repeat content, the wheat genome has always been perceived as too complex for efficient molecular studies. However, we recently constructed the first physical map of a wheat chromosome (3B). But gene mapping is still laborious in wheat because of high redundancy between the three homoeologous genomes. In contrast, in the closely related diploid species, barley, numerous gene-based markers have been developed. This study aims at combining the unique genomic resources developed in wheat and barley to decipher the organisation of gene space on wheat chromosome 3B. Results Three dimensional pools of the minimal tiling path of wheat chromosome 3B physical map were hybridized to a barley Agilent 15K expression microarray. This led to the identification of 738 barley genes with a homolog on wheat chromosome 3B. In addition, comparative analyses revealed that 68% of the genes identified were syntenic between the wheat chromosome 3B and barley chromosome 3H and 59% between wheat chromosome 3B and rice chromosome 1, together with some wheat-specific rearrangements. Finally, it indicated an increasing gradient of gene density from the centromere to the telomeres positively correlated with the number of genes clustered in islands on wheat chromosome 3B. Conclusion Our study shows that novel structural genomics resources now available in wheat and barley can be combined efficiently to overcome specific problems of genetic anchoring of physical contigs in wheat and to perform high-resolution comparative analyses with rice for deciphering the organisation of the wheat gene space.

2010-12-02 | E-TABM-1011 | biostudies-arrayexpress

GNPS - Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [nullisomic-tetrasomic lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [nullisomic-tetrasomic lines]

2023-06-23 | MSV000092256 | GNPS

GNPS - Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

2023-06-30 | MSV000092320 | GNPS

GNPS - Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines] experiment 1

2023-06-30 | MSV000092323 | GNPS

Expression profiling of wheat heads from spring wheat Chinese Spring (CS) and CS-7EL (CS with additional chromosome arm 7EL from Thinopyrum elongatum), after inoculation with either water (mock inoculation) or Fusarium graminearum

Project description:The ditelocentric addition line CS-7EL of the spring wheat (Triticum aestivum) cultivar Chinese Spring (CS) contains the long arm of the chromosome 7E from Thinopyrum elongatum (CS-7EL), which confers high resistance to fusarium head blight. It is of great interest to breeders to integrate the resistance locus (loci) from Th. elongatum into commercial wheat varieties. The objectives of this study were to identify candidate genes expressed from the 7EL chromosome of CS-7EL, to develop 7EL-specific molecular markers, and to validate their usefulness to characterize recombination between one of the group 7 chromosomes of wheat and Th. elongatum. High-throughput sequencing of Fusarium graminearum-infected and control CS and CS-7EL cDNA libraries was performed using RNA-Seq. A stepwise bioinformatics strategy was applied to assemble the sequences obtained from RNA-Seq and to create a conservative list of candidate genes expressed from the foreign chromosome 7EL. PCR primer pairs were designed and tested for 135 candidate genes. A total of 48 expressed molecular markers specific for the chromosome 7EL were successfully developed. Screening of progenies from two BC1F2 families from the cross CS-7E(7D)×2*CSph1b showed that these markers are useful to characterize recombination events between the chromosomes 7D from wheat and 7E from Th. elongatum.

2015-09-14 | GSE70797 | GEO

Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [nullisomic-tetrasomic lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [nullisomic-tetrasomic lines]

2023-06-23 | MSV000092256 | MassIVE

Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

Project description:Metabolism of halauxifen-methyl is regulated by genes on chromosome 5A of wheat (Triticum aestivum L.) [alien substitution lines]

2023-06-30 | MSV000092320 | MassIVE

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