Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1231111 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To determine microbiota composition associated with loss of KDM5 in intestine, we carried out 16S rRNA seq analyses of dissected intestine from wildtype and kdm5 mutant. [GSM2628181-GSM2628190]. A total of 78 operational taxonomic units (OTUs) were identified in the sequence data. There were about 15 genera much less abundant in kdm5 mutant compared to wildtype. The kdm5 mutant were sensitive to pathogen. To confirm the microbiota associated with loss of KDM5 in intestine, 16S rRNA of new flies were sequenced and analyzed by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China) [GSM3243472-GSM3243481]. A total of 107 operational taxonomic units (OTUs) were identified in the sequence data. There were about 20 genera much less abundant in kdm5 mutant compared to wildtype. To confirm the microbiota associated with loss of KDM5 drosophila feeding with Lactobacillus plantarum, 16S rRNA of kdm5 mutant flies were sequenced and analyzed by Novogene Bioinformatics Technology Co., Ltd. (Tianjin, China) [GSM3263522-GSM3263527]. A total of 92 operational taxonomic units (OTUs) were identified in the sequence data. To confirm the microbiota associated with KDM5 knockdown in intestine, 16S rRNA of Myo1A-Gal4TS/+ and Myo1A-Gal4TS/+;+/kdm5RNAi flies were sequenced and analyzed by Biomarker Co. Ltd. (Beijing, China). [GSM3507915-GSM3507924]. A total of 50 operational taxonomic units (OTUs) were identified in the sequence data. There was a significant different based on the genus level between two groups.
2019-02-08 | GSE98944 | GEO
Project description:Hair fibers serve as a prevalent and valuable form of biological trace evidence. Taxonomic classification of hair recovered during a criminal investigation provides crucial forensic intelligence and aids in evidence prioritisation. Traditional classification methods rely heavily on the analysis of hair shaft morphology, which requires individual analysis, can have limited taxonomic resolution, and is difficult to scale. Mass spectrometry-based proteomics can provide a biomolecular alternative by use of taxonomic amino acid polymorphisms to resolve the species of origin of trace evidence. In this study a proteomics workflow was developed for the discovery and characterization of taxonomically diagnostic hair peptides. Taxa were selected with a focus on relevance to crime scene investigation. A panel of 226 taxonomically informative peptides were identified and characterized, 209 of which are novel to this study. The biomarker panel includes 59 peptides with apparent single-species resolution, with remaining biomarkers being diagnostic in combination. The study also included 3 positive control keratin sequences where at least 2 are documented to occur in all mammals. The panel of taxa-discriminating markers was subsequently integrated into a forensic workflow, optimized for universal sample preparation and standardised LC/MS/MS data acquisition. The developed workflow was used to analyze single fur-hair shafts (20 mm), from four individuals from each species of interest. The data was further processed using a curated protein database, an efficient and validated bioinformatics workflow. The method was able to classify all hairs analysed with genus or species level resolution without prior source information.
2026-05-13 | PXD062555 | Pride
Project description:Taxonomic evaluation of the genus Citrobacter using multilocus sequence analysis
| PRJEB13613 | ENA
Project description:Improved phylogenetic resolution within the Neotropical rainforest genus Zygia (Mimoseae, Fabaceae) using phylogenomic data
| PRJEB108520 | ENA