Project description:Tandem mass spectrometry analysis of Stenotrophomonas bentonitica BII-R7 cells grown in absence or presence of 2 mM sodium selenite and sampled at three different time-points.
Project description:The Gram-negative bacterium Stenotrophomonas bentonitica BII-R7T was isolated from bentonite formations. Like other species within the genus Stenotrophomonas, strain BII-R7T possesses high tolerance to numerous heavy metals, suggesting potential for bioremediation purposes. The draft genome sequence reported here comprises 4.37 Mb with a G+C content of 66.5% and 3,796 predicted protein-coding sequences.
Project description:Color vision in Drosophila is mediated by photoreceptors R7 and R8, which express various combinations of opsins Rh3, Rh4, Rh5 and Rh6 depending on their cellular identity. Most ommatidia are classified as either “pale” or “yellow” subtypes with pale ommatidia coordinately expressing Rh3 and Rh5—in R7 and R8 cells respectively—while yellow express Rh4 and Rh6. Subtype identity is established initially in R7 photoreceptors via a stochastic mechanism then transmitted to the R8 via an inductive signal to ensure paired opsin expression. To identify factors that may be involved in this process, we used RNA-Seq to detect genes that are differentially expressed in sevenless mutant retinas at 40 hours after puparium formation. Since loss of sevenless prevents R7 recruitment and specification, we reasoned that this approach would allow us to identify genes that are enriched in R7 cells during this critical time point. Furthermore, since it has previously been established that in the absence of R7 most R8s will adopt the yellow Rh6-expressing identity, this gives us the opportunity to identify genes which may inductive mechanism occurring in R8 cells.
Project description:We sought to determine how a cystic fibrosis isolate of Stenotrophomonas maltophilia responds to relevant pH gradients (pH 5, 7, and 9) by growing the bacterium in phosphate buffered media and conducting RNAseq experiments. Our data suggests acidic conditions are stressful for strain FLR19, as it responded by increasing expression of stress-response and antibiotic-resistance genes.