GEO accession GSE291177 is currently private and is scheduled to be released on Nov 09, 2025.
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1232147 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The aim of this study was to analyse the transcriptional regulation of UVB-induced ocular rosacea model on the meibomian glands of rats that overexpress the human NRC32 gene coding for mineralocorticoid receptor (P1.hMR) and age- and sex- matched wild type (WT) littlemates. 10-12 weeks old female Wild-Type (WT) and P1.hMR rats were subjected to 5-days UVB irrdiation on eyelids (UVB_WT: n = 3, UVB_P1.hMR: n = 5), together with age- and sex- matched rats without UVB-induced model (Control_WT: n = 4, Control_P1.hMR: n = 5), they were euthanized and the meibomian glands were dissected for RNA-sequencing. Total RNA samples were sequenced at the iGenSeq transcriptomic platform of the Brain and Spine Institute (ICM, Paris, France). RNA quality was checked by capillary electrophoresis and RNA integrity numbers (RIN) ranging from 8.8 to 9.3 was accepted for library generation. Quality of raw data was assessed using FastQC. Poor quality sequences and adapters were trimmed or removed with fastp, using default parameters, to retain only good quality paired reads. Illumina DRAGEN bio-IT Plateform (v3.8.4) was used for mapping on rn7 reference genome. Library orientation, library composition and coverage along transcripts were checked with Picard tools. Differential analysis has been also conducted with DESeq2. Multiple hypothesis adjusted p-values were calculated with the Benjamini-Hochberg procedure to control FDR. FDR threshold was set at 0.05, log2 fold-change threshold was set at 0.5. We identified genes differentially regulated in UVB-treated WT as compared to Control WT, and genes differentially regulated in UVB-treated P1.hMR rats as compared to Control P1.hMR.
2026-02-25 | GSE291177 | GEO
Project description:We have developed a rat model of UVB-induced ocular surface and eyelid damages mimicking ocular rosacea, a common chronic inflammatory and neurovascular ocular surface disease associated with meibomian gland dysfunction. Rat eyelids were exposed to UVB for 5 minutes daily (300mJ/cm2) for 5 days. UVB-exposed rats displayed altered meibocyte activity and lipid production, ductal epithelial hyper-keratinization, apoptosis, mitochondrial dysfunction and oxidative stress. Corneal epithelial defects, barrier disruption and abnormal innervation occurred 2 weeks after UVB exposure, subsequent to meibomian gland dysfunction. The aim of this study was to investigate the transcriptomic signature associated with meibomian gland dysfunction induced by UVB in rats. We revealed pathways related to keratinization, cell cycle and proliferation, DNA damage and repair, inflammation, immune responses, oxidative responses, and fatty acid metabolism. These regulations supported the observed functional changes of meibomian gland and were consistent with the transcriptomic signature of human meibomian gland dysfunction.
2025-12-31 | GSE277020 | GEO
Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction. Total RNA was isolated from normals (n=6) and patients with meibomian disease (n=6). The RNA was then used in conjuction with HumanHT-12 v3 Expression BeadChips to assay differential gene expression between normal and diseased meibomian glands.
2010-12-31 | E-GEOD-17822 | biostudies-arrayexpress
Project description:RNA-sequencing of young and old mouse eyelids identified a number of signaling pathways, including FGF and Wnt that were altered in meibomian glands and conjunctiva of aging mice.
2016-03-20 | GSE78499 | GEO
Project description:The objective of this study is to identify human meibomian gland genes that may promote the development and/or progression of meibomian gland dysfunction.
2010-12-31 | GSE17822 | GEO
Project description:Effect of eyelid UVB irradiation on gene expression of rat meibomian gland
| PRJNA1160230 | ENA
Project description:Rosacea is a common chronic inflammatory skin disease of unknown etiology. Our knowledge about an involvement of the adaptive immune system is very limited. We performed detailed transcriptome analysis, qRT-PCR, and quantitative immunohistochemistry on facial biopsies of rosacea patients, classified according to their clinical subtype. As controls, we used samples from healthy controls. Our study shows significant activation of the immune system in all subtypes of rosacea, characterizing erythematotelangiectatic rosacea (ETR) already as a disease with significant influx of proinflammatory cells. The T cell response is dominated by Th1/Th17-polarized immune cells, as demonstrated by significant upregulation of IFNγ or IL-17, for example. Chemokine expression patterns support a Th1/Th17 polarization profile of the T cell response. Macrophages and mast cells are increased in all three subtypes of rosacea, while neutrophils reach a maximum in papulopustular rosacea. Our studies also provide evidence for activation of plasma cells with significant antibody production already in ETR, followed by a crescendo pattern towards phymatous rosacea. In sum, Th1/Th17 polarized inflammation and macrophage infiltration is an underestimated hallmark in all subtypes of rosacea. Therapies directly targeting the Th1/Th17 pathway are promising candidates in the future treatment of this skin disease.
2015-06-01 | GSE65914 | GEO
Project description:Rosacea is a common chronic inflammatory skin disease of unknown etiology. Our knowledge about an involvement of the adaptive immune system is very limited. We performed detailed transcriptome analysis, qRT-PCR, and quantitative immunohistochemistry on facial biopsies of rosacea patients, classified according to their clinical subtype. As controls, we used samples from healthy controls. Our study shows significant activation of the immune system in all subtypes of rosacea, characterizing erythematotelangiectatic rosacea (ETR) already as a disease with significant influx of proinflammatory cells. The T cell response is dominated by Th1/Th17-polarized immune cells, as demonstrated by significant upregulation of IFNγ or IL-17, for example. Chemokine expression patterns support a Th1/Th17 polarization profile of the T cell response. Macrophages and mast cells are increased in all three subtypes of rosacea, while neutrophils reach a maximum in papulopustular rosacea. Our studies also provide evidence for activation of plasma cells with significant antibody production already in ETR, followed by a crescendo pattern towards phymatous rosacea. In sum, Th1/Th17 polarized inflammation and macrophage infiltration is an underestimated hallmark in all subtypes of rosacea. Therapies directly targeting the Th1/Th17 pathway are promising candidates in the future treatment of this skin disease. Total of 58 chips. 19 patients and 10 healthy volunteers
2015-06-01 | E-GEOD-65914 | biostudies-arrayexpress
Project description:Our objective was to determine the nature and extent of androgen regulation of gene expression in the female lacrimal, meibomian,and submandibular glands, and to explore the degree to which this control is the same as in male glands. Keywords: Hormone treatment
2006-01-10 | GSE3995 | GEO