Project description:Thyroid cancer is the most prevalent malignancy of the endocrine system. We and others have shown that several microRNAs, which are ~21-24nt post-transcriptional gene regulators, are aberrantly expressed in anaplastic thyroid cancer (ATC) and papillary thyroid cancer (PTC) tissues and cell lines. In the cell, miRNAs are bound to Argonaute (AGO) proteins as low molecular weight RNA Induced Silencing Complexes (LMW-RISCs) that can then assemble into high molecular weight RISCs (HMW-RISCs) that also include additional proteins and mRNA. In this study, we sought to analyze the association of miRNAs across RISC complexity in ATC and PTC. For both ATC and PTC lines, miRNAs were enriched in HMW-RISC and LMW-RISC fractions compared with intermediate fractions or very low molecular weight (AGO-poor) fractions. Furthermore, 60% of all miRNAs were more abundant in LMW-RISC versus HMW- RISC fractions by ~2-4 fold. Surprisingly, miR-21-5p, one of the most abundant miRNAs in both ATC and PTC lines, was consistently one the least abundant miRNAs in HMW-RISC and the most enriched miRNA in LMW-RISC fractions. These findings suggest that miR-21 may be uniquely distributed in RISCs relative to other miRNAs. Furthermore, the methodology described here is a useful way to assess the relative magnitude of miR-21association with HMW and LMW-RISCs and may help to reveal the true roles of this miRNA in thyroid cancer development, progression, and treatment.
Project description:The distribution of individual miRNA species between high-molecular weight and low-molecular weight RISC complexes by small RNA sequencing in thyroid cancer cell lines
Project description:In this study, the effects of three high molecular weight phthalates (DINP, DIDP, DPHP) and their metabolites (MHINP, MCINP, OHMPHP) were investigated in THP-1 macrophages. For this purpose, the phosphoproteome was evaluated.
Project description:In this study, the effects of three high molecular weight phthalates (DINP, DIDP, DPHP) and their metabolites (MHINP, MCINP, OHMPHP) were investigated in THP-1 macrophages. For this purpose, untargeted proteomics was applied.
Project description:This clinical trial studies the effectiveness of a web-based cancer education tool called Helping Oncology Patients Explore Genomics (HOPE-Genomics) in improving patient knowledge of personal genomic testing results and cancer and genomics in general. HOPE-Genomics is a web-based education tool that teaches cancer/leukemia patients, and patients who may be at high-risk for developing cancer, about genomic testing and provide patients with information about their own genomic test results. The HOPE-Genomics tool may improve patient’s genomic knowledge and quality of patient-centered care. In addition, it may also improve education and care quality for future patients.
Project description:In this study, the effects of three high molecular weight phthalates (DINP, DIDP, DPHP) and their metabolites (MHINP, MCINP, OHMPHP) were investigated in THP-1 macrophages. For this purpose, the redoxome, including overall as well as reversible cysteine oxidation, was evaluated.
Project description:Interventions: low-molecular-weight heparin after surgery
no treatment
Primary outcome(s): The efficacy of low-molecular-weight heparin to prevent deep venous thrombosis after surgery for colorectal cancer
Study Design: Parallel Randomized
Project description:High-molecular-weight glutenin subunits (HMW-GS) play an essential role in the end-use quality of wheat (Triticum aestivum L.). We developed a targeted liquid chromatography-tandem mass spectrometry method in parallel reaction monitoring (LC-MS/MS-PRM) to detect and differentiate wheat HMW-GS in German wheat cultivars with known (37 cultivars) and unknown (58 cultivars) composition. The newly developed method is suitable to unambiguously identify Ax1, Ax2*, Bx6, Bx14, Dx2, Dx5, Dy10, Dy12, as well as any absence of Ax, but cannot distinguish Bx7 and Bx17 and identify the variant of By due to high sequence identity to Dy and within By. The method is further suited to clearly conclude, if the sample is a mixture of at least two cultivars or consists of only one cultivar. In comparison to gel-based methods (SDS-PAGE), UV-detection after LC (RP-HPLC-UV) and MS of intact proteins (MALDI-TOF-MS), LC-MS/MS has a high resolution, is less biased by interpretation and provides more insights on molecular level. The used procedure can be applied to expand the LC-MS/MS-PRM method for more HMW-GS or even to other wheat proteins, e.g., low-molecular-weight glutenin subunits (LMW-GS), in future. This study describes the first MS-based method on peptide level for the differentiation of wheat HMW-GS.