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Expression data of circTMEM45A knockdown in KYSE-150 cell line

ABSTRACT: Expression data of circTMEM45A knockdown in KYSE-150 cell line

PROVIDER: PRJNA1233263 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32610200_1.fastq.gz	Fastqsanger.gz
	SRR32610200_2.fastq.gz	Fastqsanger.gz
	SRR32610201_1.fastq.gz	Fastqsanger.gz
	SRR32610201_2.fastq.gz	Fastqsanger.gz
	SRR32610202_1.fastq.gz	Fastqsanger.gz

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Expression data of circTMEM45A knockdown in KYSE-150 cell line

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Expression data from KYSE-150-shCtrl and KYSE-150-shCDKL3 cells

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Expression data from KYSE-150-shCtrl and KYSE-150-shCDKL3 cells

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Expression data comparing KYSE-140 ESCC cell line CD90+ and CD90- cells

Project description:To identify candidate genes involved in enhanced tumorigenicity and metastasis of CD90+ esophageal tumor-initiating cells. The esophageal squamous carcinoma cell (ESCC) cell line KYSE-140 was sorted into CD90+ and CD90- populations by flow cytometry. Total RNA was analyzed on Affymetrix Human Genome U133 Plus GeneChip 2.0 arrays.

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Next generation sequencing of esophageal squamous cell carcinoma (ESCC) cell line KYSE-180 single cell transcriptomes for radio-resistance analysis

Project description:Purpose: Single cell RNA-seq could observe heterogeneity of cell transcriptomes. The goals of this study are to compare the changes of ESCC cell line KYSE-180 RNA profiles in response to different doses of radiotherapy and to analyze the radio-resistance related genes.

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Phosphoproteomics of NEEC and KYSE cell lines

Project description:Phosphoproteomics of NEEC and KYSE cell line KYSE410 and KYSE450

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RNAseq profiling of eSCC human cell line (KYSE-410) treated with pharmacological inhibitors of SHIP2

Project description:In our study, we used mRNA sequencing to investigate the effect of SHIP2 inhibition on the transcriptome of KYSE-410 cells.

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MEIS1 knockdown may promote differentiation of esophageal squamous carcinoma cell line KYSE-30.

Project description:BackgroundMEIS1 (Myeloid ecotropic viral integration site 1), as a homeobox (HOX) transcription factor, has a dual function in different types of cancer. Although numerous roles are proposed for MEIS1 in differentiation, stem cell function, gastrointestinal development and tumorigenesis, the involved molecular mechanisms are poor understood. Our aim in this study was to elucidate the functional correlation between MEIS1, as regulator of differentiation process, and the involved genes in cell differentiation in human esophageal squamous carcinoma (ESC) cell line KYSE-30.MethodsThe KYSE-30 cells were transduced using recombinant retroviral particles containing specific shRNA sequence against MEIS1 to knockdown MEIS1 gene expression. Following RNA extraction and cDNA synthesis, mRNA expression of MEIS1 and the selected genes including TWIST1, EGF, CDX2, and KRT4 was examined using relative comparative real-time PCR.ResultsRetroviral transduction caused a significant underexpression of MEIS1 in GFP-hMEIS1 compared to control GFP cells approximately 5.5-fold. While knockdown of MEIS1 expression caused a significant decrease in EGF and TWIST1 mRNA expression, nearly -8- and -12-fold respectively, it caused a significant increase in mRNA expression of differentiation markers including KRT4 and CDX2, approximately 34- and 1.14-fold, correspondingly.ConclusionMEIS1 gene silencing in KYSE-30 cells increased expression of epithelial markers and decreased expression of epithelial-mesenchymal transition (EMT) marker TWIST1. It may highlight the role of MEIS1 in differentiation process of KYSE-30 cells. These results may confirm that MEIS1 silencing promotes differentiation and decreases EMT capability of ESC cell line KYSE-30.

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RNAseq profiling of eSCC human cell line (KYSE-410) knocked-out for SHIP2 using CRISPR/Cas9 technology

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