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ABSTRACT: Translation of bi-directional transcripts enhances MHC-I peptide diversity

PROVIDER: PRJNA1235990 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32731600_1.fastq.gz	Fastqsanger.gz
	SRR32731600_2.fastq.gz	Fastqsanger.gz
	SRR32731601_1.fastq.gz	Fastqsanger.gz
	SRR32731601_2.fastq.gz	Fastqsanger.gz
	SRR32731602_1.fastq.gz	Fastqsanger.gz

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Translation of bi-directional transcripts generates peptides for the MHC-I pathway.

Project description:Antisense transcripts play an important role in generating regulatory non-coding RNAs but if these transcripts are also translated and for what purpose is yet poorly understood. Isolation of polysome fractions followed by RNA sequencing and mass spectrometry analysis of a six frame data-base revealed nascent peptides originating from different reading frames of bi-directional transcripts. Two peptides originating from the antisense strand stimulated the proliferation of CD8+ T cells when presented to peripheral blood mononuclear cells (PBMCs) from nine healthy donors. An antigenic peptide derived from the reverse strand of two cDNA constructs was presented on MHC-I molecules and induced CD8+ T cell activation. These results demonstrate that three frame translation of bi-directional transcripts generate antigenic peptide substrates for the immune system. This discovery holds significance for better understanding the origin of self-discriminating peptide substrates for the MHC-I pathway and for enhancing immune-based therapies against infected or transformed cells.

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