Project description:The proteome of human plaque, fibrous cap, core, and media was unveiled using fully automated sample preparation platform AccelerOme and cutting-edge DIA mass spectrometry.
Project description:Cell polarity is used to guide asymmetric divisions and create morphologically diverse cells. We find that two oppositely oriented cortical polarity domains present during the asymmetric divisions in the Arabidopsis stomatal lineage are reconfigured into polar domains marking ventral (pore-forming) and outward facing domains of maturing stomatal guard cells. Proteins that define these opposing polarity domains were used as baits in miniTurboID-based proximity labeling. Among differentially enriched proteins we find SOSEKIs and their effector ANGUSTIFOLIA, protein kinase CKII and LC8-type DYNEIN LIGHT CHAIN1 as polar scaffolds. Using AI-facilitated protein structure prediction models, we identify their potential interaction interfaces. Functional and localization analysis of polarity protein OPL2 and its newly discovered partners suggest a positive interaction with mitotic microtubules and a potential role in cytokinesis. This combination of cutting-edge proteomics and structural modeling with live cell imaging provides insights into how polarity is rewired in different cell types and cell cycle stages.
Project description:In this study, a series of cutting-edge technologies such as protein extraction, enzyme digestion, liquid chromatography-mass spectrometry tandem analysis and bioinformation analysis were organically combined to study the quantitative proteome of samples.
Project description:In this study, the samples were subjected to quantitative proteomic studies through the application of a series of cutting-edge techniques, including protein extraction, enzyme digestion, liquid chromatography-mass spectrometry tandem analysis, and bioinformatic analysis.
Project description:This study conducted a quantitative proteomic analysis of haliotis discus hannai samples by combining cutting-edge technologies such as protein extraction, enzyme digestion, liquid chromatography-mass spectrometry tandem analysis, and bioinformatics analysis.
