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Phosphorylation of CSDE1 drives ribosome interactions with implications for cancer malignancy

ABSTRACT: Phosphorylation of CSDE1 drives ribosome interactions with implications for cancer malignancy

PROVIDER: PRJNA1236333 | ENA |

REPOSITORIES: ENA

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Phosphorylation of CSDE1 drives ribosome interactions with implications for cancer malignancy

Project description:The RNA-binding protein CSDE1 is a key regulator of mRNA stability and translation in a broad spectrum of biological processes. We have previously shown that CSDE1 functions as an oncoprotein in melanoma, where it promotes invasion and metastasis, whereas it behaves as a tumour suppressor in squamous cell carcinoma, a tumor derived from keratinocytes. The reasons underlying these context-specific behaviours are unknown. To identify melanoma-specific vulnerabilities, we have compared CSDE1 protein isoforms and post-translational modifications in melanoma cells and keratinocytes, as well as in cells of different tumorigenic potential. Combining Nanopore sequencing with two-dimensional gel electrophoresis and proteomics, we identify one major CSDE1 isoform expressed in melanoma cells and patient samples and find that CSDE1 phosphorylation and interactions with ribosomes represent an early event in cellular transformation, correlating with changes in the subcellular localization of CSDE1. Our data show that CSDE1 phosphorylation promotes de novo interactions of CSDE1 with ribosomes, uncovering a specific vulnerability of melanoma cells that might be harnessed for therapeutic intervention.

2025-08-13 | GSE292019 | GEO

Ribosome profiling of UNR (CSDE1) KD

Project description:Investigation of the effects of UNR (CSDE1) knock-down on translation in Melanoma Cells using Ribosome Profiling

2016-11-07 | E-MTAB-3815 | biostudies-arrayexpress

Sciatic nerve injury-responsive differential CSDE1 association efficiency of ribosomal protein coding mRNAs

Project description:Gpr151 is an injury-responsive gene that promotes axon regeneration through its interaction with the 5'UTR region of its mRNA transcripts and the RNA-binding protein CSDE1. We found that knocking down or overexpressing the 5'UTR of Gpr151 changes the ribosomal protein mRNA regulon regulated by CSDE1. After the sciatic nerve injury, the association between the ribosomal protein mRNA regulon and CSDE1 was enhanced at dorsal root ganglions(DRGs), but this enhancement was not observed in the AAV-shGpr151 injected mice. Overexpression of 5'UTRmut, which has increased binding affinity to CSDE1, enhanced the ribosomal protein mRNA regulon and its association with CSDE1 in embryonic DRG neurons.

2023-08-18 | GSE236910 | GEO

RPL12/uL11 phosphorylation regulates translation during mitosis [Ribosome profiling]

Project description:RPL12/uL11 phosphorylation regulates translation during mitosis [Ribosome profiling]

| PRJNA445183 | ENA

Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [RNA-Seq]

Project description:We show that the RNA-binding protein CSDE1/UNR promotes oncogene-induced senescence (OIS) in primary mouse keratynocytes challenged by over-expression of H-RASv12. Depletion of CSDE1 leads to senescence bypass, cell immortalization and tumor formation. Combining individual nucleotide cross-linking and immunoprecipitation (iCLIP), RNA-seq and polysome profiling followed by functional studies, we identify targets regulated by CSDE1 and uncover the downstream molecular mechanisms.

2022-01-11 | GSE159999 | GEO

Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [polysome profiling]

2022-01-11 | GSE159998 | GEO

Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [i-CLIP]

2022-01-11 | GSE159997 | GEO

iCLIP transcriptome-wide mapping of UNR (CSDE1) binding sites

Project description:Analysis of UNR (CSDE1) binding to RNA targets by iCLIP transcriptome-wide mapping.

2016-11-07 | E-MTAB-3818 | biostudies-arrayexpress

Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [RNA-Seq-sh]

2022-01-11 | GSE160000 | GEO

Ribosome profiling uncovers selective mRNA translation associated with eIF2 phosphorylation in erythroid progenitors

Project description:The aim of this study is to investigate how eIF2 phosphorylation affects mRNA translation in erythroblasts. Ribosome profiling combined with RNA sequencing was used to determine translation initiation sites and ribosome density on individual transcripts.

2019-02-01 | GSE110618 | GEO

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