Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1236535 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR32707300_1.fastq.gz | Fastqsanger.gz | |||
| SRR32707300_2.fastq.gz | Fastqsanger.gz | |||
| SRR32707301_1.fastq.gz | Fastqsanger.gz | |||
| SRR32707301_2.fastq.gz | Fastqsanger.gz | |||
| SRR32707302_1.fastq.gz | Fastqsanger.gz |
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Project description:Time-restricted feeding improves metabolic health independently of dietary macronutrient composition or energy restriction. To understand the mechanisms underpinning the effects of time-restricted feeding, we investigated the metabolic and transcriptomic profile of skeletal muscle and serum samples from 11 overweight/obese men. In muscle, 4-10% of transcripts and 14% of metabolites were periodic, with the amplitude of the metabolites lower after time-restricted feeding. Core clock genes were unaltered by either intervention, while time-restricted feeding induced rhythmicity of genes related to lipid and amino acid transport. In serum, 49-65% of the metabolites had diurnal rhythms across both conditions, with the majority being lipids. Time-restricted feeding shifted the skeletal muscle metabolite profile from predominantly lipids to amino acids. Our results show time-restricted feeding differentially affects the amplitudes and rhythmicity of serum and skeletal muscle metabolites, and regulates the rhythmicity of genes controlling lipid and amino acid transport, without perturbing the core clock.
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Project description:This study investigated the effects of dietary sulfur amino acid restriction (SAAR) on the mouse liver transcriptome. We fed wild-type (C57Bl/6J) male mice either a control (Ctrl) or SAAR diet on either a regular-fat (RF) or high-fat HF) background for seven days. After seven days, liver samples were collected, among other tissues, and processed for RNA-sequencing. We then analyzed the changes in the transcriptome, comparing the two different Ctrl vs. SAAR comparisons against each other to identify how HF might impact the effect of SAAR on the hepatic transcriptome. Additionally, this project also involved generating proteomics data which we used to compare the similarities and differences between transcriptomic and proteomic changes in response to the different dietary treatments. We find that SAAR, independent of diet, produces a robust realignment of the transcriptome after seven days of SAAR. In contrast, proteome-wide changes were less well-aligned.
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| PRJNA823291 | ENA