Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1238254 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative transcriptomic profile of quorum sensing regulated genes in Hafnia alvei FB1
2016-12-30 | GSE93000 | GEO
Project description:Dataset 1 contains bacterial-fungal monocultures and tripartite co-cultures in which Scopulariopsis sp. JB370 are grown with 1) Hafnia alvei JB232 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. Dataset 2 contains bacterial-fungal tripartite co-cultures in which Penicillium solitum #12 are grown with 1) Glutamicibacter arilaitensis JB182 or Brevibacterium linens JB5 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. All cultures from each dataset were grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking.
2023-03-08 | MSV000091440 | MassIVE
Project description:Hafnia alvei H4 is a bacteria subject to regulation by N-acyl-l-homoserine lactone (AHL)-mediated quorum sensing system and is closely related to the corruption of instant sea cucumber. Studying the effect of Hafnia alvei H4 quorum sensing regulatory genes on AHLs is necessary for the quality and preservation of instant sea cucumber. In this study, the draft genome of H. alvei H4, which comprises a single chromosome of 4,687,151 bp, was sequenced and analyzed and the types of AHLs were analyzed employing thin-layer chromatography (TLC) and LC-MS/MS. Then the wild-type strain of H. alvei H4 and the luxI/R double mutant (ΔluxIR) were compared by transcriptome sequencing (RNA-seq). The results indicate that the incomplete genome sequence revealed the presence of one quorum-sensing (QS) gene set, designated as lasI/expR. Three major AHLs, N-hexanoyl-L-homoserine lactone (C6-HSL), N-butyryl-L-homoserine lactone (C4-HSL), and N-(3-oxo-octanoyl)-L-homoserine lactone (3-oxo-C8-HSL) were found, with C6-HSL being the most abundant. C6-HSL was not detected in the culture of the luxI mutant (ΔluxI) and higher levels of C4-HSL was found in the culture of the luxR mutant (ΔluxR), which suggested that the luxR gene may have a positive effect on C4-HSL production. It was also found that AHL and QS genes are closely related in the absence of luxIR double deletion. The results of this study can further elucidate at the genetic level that luxI and luxR genes are involved in the regulation of AHL.
2019-10-10 | GSE137815 | GEO
Project description:Theaflavin-3,3´-digallate (TF3) exhibits anti-quorum sensing (QS) properties against Hafnia alvei H4, a bacterium commonly found in spoiled aquatic products, by interacting with LuxI and reducing AHL concentration. This study investigated the extensive changes in the transcriptional and metabolic profiles of H. alvei H4 induced by TF3 as a QS inhibitor (QSI). The results demonstrated that a subminimal inhibitory concentration of TF3 (31.25 µM) disrupted the QS system of H. alvei H4 by down-regulating the expression of genes involved in QS and decreasing AHL concentrations. The dysfunction of the QS system in H. alvei H4 exacerbated oxidative stress, which in turn altered membrane composition, increased membrane permeability, and disrupted energy metabolism, amino acid metabolism, and nucleotide metabolism, ultimately contributing to the anti-QS effect. Subsequent validation through real-time fluorescence quantitative PCR and enzyme assays corroborated the sequencing results. These findings enhance our understanding of the comprehensive anti-QS effects induced by TF3, which may facilitate its future development and application in ensuring food safety.
2025-08-01 | GSE276235 | GEO
Project description:Brevibacterium aurantiacum overview
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Project description:Investigation of the interactions between Brevibacterium aurantiacum strains and Hafnia alvei in a lab scale mini-cheese model using high-throughput RNA sequencing
| PRJEB30420 | ENA