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The positionally conserved lncRNA DANCR is an essential regulator of zebrafish development and a human melanoma oncogene

ABSTRACT: The positionally conserved lncRNA DANCR is an essential regulator of zebrafish development and a human melanoma oncogene

PROVIDER: PRJNA1238859 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32791434_1.fastq.gz	Fastqsanger.gz
	SRR32791434_2.fastq.gz	Fastqsanger.gz
	SRR32791435_1.fastq.gz	Fastqsanger.gz
	SRR32791435_2.fastq.gz	Fastqsanger.gz
	SRR32791436_1.fastq.gz	Fastqsanger.gz

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The positionally conserved lncRNA DANCR is an essential regulator of zebrafish development and a human melanoma oncogene

Project description:Long non-coding RNAs (lncRNAs) play crucial roles in regulating gene expression. Some are essential for organismal development and physiology, and they can contribute to diseases such as cancer. Whilst most lncRNAs exhibit little sequence similarity, conservation of lncRNA transcription relative to neighbouring protein-coding genes suggests potential functional significance. Nevertheless, most positionally equivalent lncRNAs are uncharacterized and it remains unclear whether they exert similar roles in distant species. Here, we identified syntenic melanoma-associated lncRNAs predicted to be components of the MITF gene regulatory network in human melanoma, with positionally equivalent transcripts in zebrafish. Among these, we prioritized Differentiation Antagonizing Non-Protein Coding RNA (DANCR), a cancer-associated lncRNA critical for maintaining somatic progenitor cells in human models, for functional investigation. Dancr is a multi-exonic, cytoplasmically enriched lncRNA transcribed from syntenic regions in the human and zebrafish genomes. MITF and c-MYC, key melanoma transcription factors, regulate human DANCR expression and melanoma patients with high DANCR display significantly decreased survival. DANCR is a melanoma oncogene that controls cancer-associated gene expression networks and promotes human melanoma cell proliferation and migration. Zebrafish dancr is dynamically expressed across multiple different cell types in the developing embryo, regulates genes involved in cell death, and is essential for embryonic development. Our work suggests that cancer-critical lncRNAs such as Dancr, expressed from similar regions in vertebrate genomes, may regulate related genes and processes involved in both embryonic development and tumorigenesis across species.

2025-03-25 | GSE292491 | GEO

Role of MYC target lncRNA DANCR in cancer

Project description:Expression profiling by array Effects of DANCR siRNA on gene expression

2016-12-05 | GSE76176 | GEO

RNA-seq in SUNE-1 cells after downregulation of DANCR expression

Project description:To explore whether DANCR plays a role in nasopharyngeal carcinoma tumorigenesis, a RNA-seq analysis was performed to compare the gene expression profiles of DANCR siRNA and control groups.

2019-07-20 | GSE117415 | GEO

Altered expression of genes in DANCR knockdown bladder cancer cells

Project description:The prognosis for bladder cancer (BCa) patients with lymph node (LN) metastasis is forlorn and restrainedly improved by current treatment. DANCR is reported to play a role in prostate cancer and liver cancer. However, its function and underlying mechanism in BCa remains unknown. The goal of this study is to identify the target genes of DANCR in bladder cancer. Our results indicate that the genes regulated by DANCR are involved in a variety of biological functions, such as proliferation and metastasis.

2019-09-01 | GSE119639 | GEO

A conserved microRNA signature related to cellular transformation by the RAS oncogene

Project description:A conserved microRNA signature related to cellular transformation by the RAS oncogene

| PRJNA208859 | ENA

A conserved microRNA signature related to cellular transformation by the RAS oncogene (miRNA)

Project description:A conserved microRNA signature related to cellular transformation by the RAS oncogene (miRNA)

| PRJNA208860 | ENA

lncRNAs and mRNAs expression profile in liver diseases including HCC

Project description:Sustained hepatic inflammation can contribute to cancer initiation and progression via tumor-initiating cell expansion, but underlying mechanisms remains unknown. Expression profiles of lncRNAs/mRNAs were measured in normal, chronic hepatitis, cirrhotic and cancerous livers. We found abundant disease-related lncRNAs/mRNAs deregulated across different stages of inflammation-triggered liver disease and refined a transformation gene signature to distinguish pathological liver tissues. Amongst this signature, a conserved lncRNA DANCR was silenced in normal adult liver, but overexpressed in fetal and cancerous livers. Remarkably, increased DANCR significantly correlates with poor prognosis in multiple-center cohorts and is directly induced by inflammatory pathways including NF-ÎºB and STAT3. DANCR could suppress cell differentiation and drive expansion of tumor-initiating cells, leading to chemoresistance. Moreover, in vitro and in vivo inhibition confirms the significance of DANCR as a therapeutic target when combined with other chemotherapy. We illustrate the role of DANCR relies on the regulation of CTNNB1 in a novel miRNA-blocking manner. Our studies reveal the expression of lncRNAs/mRNAs in normal and pathological livers and suggest the importance of oncofetal lncRNA DANCR in inflammation-induced malignant transformation, offering a potential prognostic marker and a therapeutic target for HCC. In the study, 10 normal livers (NL), 10 chronic inflammatory livers (IL), 10 cirrhotic livers (CL), 13 early HCC (eHCC) and 13 advanced HCC (aHCC) samples were profiled their lncRNA/mRNA expression

2014-01-22 | E-GEOD-54238 | biostudies-arrayexpress

A conserved microRNA signature related to cellular transformation by the RAS oncogene (Affymetrix mRNA)

Project description:A conserved microRNA signature related to cellular transformation by the RAS oncogene (Affymetrix mRNA)

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LncRNA DANCR Promotes Lung Cancer by Sequestering miR-216a.

Project description:BackgroundLong noncoding RNAs (lncRNAs) are a new class of cancer regulators. Here, we aimed to investigate the diagnostic and therapeutic values of an lncRNA, differentiation antagonizing noncoding RNA (DANCR), in lung cancer.MethodsReal-time polymerase chain reaction was used to compare DANCR levels in normal and cancerous lung tissues as well as lung cancer cells. Lentiviral transduction was used to induce DANCR overexpression or silencing in vitro, followed by monitoring cell proliferation, colony formation, and changes in microRNA-216a (miR-216a) expression. DANCR-specific small hairpin RNA transduction was used to establish cells with stable DANCR knockdown, and silenced cells were used to initiate lung tumor xenografts, followed by monitoring tumor growth.ResultsDANCR upregulation was seen in lung cancer, particularly in high-grade lung cancer tissues and aggressive cancer cells. Ectopic DANCR expression induced lung cancer cell proliferation and colony formation, whereas DANCR silencing induced opposing effects. The miR-216a level in cancer cells was negatively correlated with DANCR expression. The DANCR knockdown reduced the growth of tumor xenografts in vivo.ConclusionDANCR upregulation is a potential indicator of aggressive lung cancer. Silencing of DANCR has great potential as a potent therapeutic strategy in lung cancer.

| S-EPMC6852365 | biostudies-literature

Altered expression of genes in DANCR knockdown bladder cancer cells

Project description:Altered expression of genes in DANCR knockdown bladder cancer cells

| PRJNA489841 | ENA