Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Uncovering gene regulatory differences between human and chimpanzee neural cells [CUT&Tag]

ABSTRACT: Uncovering gene regulatory differences between human and chimpanzee neural cells [CUT&Tag]

PROVIDER: PRJNA1239914 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Uncovering gene regulatory differences between human and chimpanzee neural cells

Project description:Uncovering gene regulatory differences between human and chimpanzee neural cells

| PRJNA1263045 | ENA

Uncovering gene regulatory differences between human and chimpanzee neural cells

Project description:Uncovering gene regulatory differences between human and chimpanzee neural cells

| PRJNA1263046 | ENA

Uncovering gene regulatory differences between human and chimpanzee neural cells [CUT&Tag]

Project description:A major challenge in human evolutionary biology is to pinpoint the genetic differences that underlie phenotypic changes in the human lineage, such as increased neuron number and a prolonged period of neuronal maturation. Here, we used human-chimpanzee tetraploid cells to distinguish changes in gene expression due to cis-acting sequence variants that change local gene regulation, from trans expression changes due to species differences in the cellular environment, in neural progenitor cells and excitatory neurons. Using a CRISPR inhibition screen, transcription factor motif analyses, and downstream single-locus studies, we further characterized cis-acting gene regulatory differences, including changes to FOS, TNIK, FOSL2, and MAZ, that influence neurogenesis and neuronal maturation. This study identifies specific genomic changes that may contribute to human neural specializations and provides a general framework for understanding genetic differences that underlie human traits.

2025-08-12 | GSE292529 | GEO

Uncovering gene regulatory differences between human and chimpanzee neural cells [scRNA-seq]

Project description:Uncovering gene regulatory differences between human and chimpanzee neural cells [scRNA-seq]

| PRJNA1240349 | ENA

Uncovering gene regulatory differences between human and chimpanzee neural cells [ATAC-seq]

Project description:Uncovering gene regulatory differences between human and chimpanzee neural cells [ATAC-seq]

| PRJNA1239912 | ENA

Uncovering gene regulatory differences between human and chimpanzee neural cells [RNA-seq]

Project description:Uncovering gene regulatory differences between human and chimpanzee neural cells [RNA-seq]

| PRJNA1240812 | ENA

Uncovering gene regulatory differences between human and chimpanzee neural cells [scRNA-seq]

2025-08-12 | GSE292632 | GEO

Uncovering gene regulatory differences between human and chimpanzee neural cells [ATAC-seq]

2025-08-12 | GSE292528 | GEO

Uncovering gene regulatory differences between human and chimpanzee neural cells [RNA-seq]

2025-08-12 | GSE292688 | GEO

Massively parallel dissection of human accelerated regions in human and chimpanzee neural progenitors [chimpanzee]

Project description:Human accelerated regions (HARs) are evolutionarily conserved sequences that acquired human-specific nucleotide changes and reside in genomic regions associated with unique human traits and disease. The majority of HARs (96%) are noncoding, a few of which have been shown to be functional enhancers. Here, we comprehensively tested human and chimpanzee sequences of HARs (N=714) for enhancer activity using a lentivirus-based massively parallel reporter assay (lentiMPRA) in human and chimpanzee iPSC derived neural progenitors at two differentiation time points. We found that 43% (306/714) function as enhancers and over two-thirds (204/306) showed consistent differences in activity between human and chimpanzee sequences across conditions. We also tested all possible permutations of substitutions in seven HARs and found significant positive and negative interactions. Our study provides a comprehensive resource of functional neurodevelopmental HAR enhancers and shows that multiple interacting sites drive evolutionary activity differences.

2018-02-17 | GSE110759 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data