Project description:The current research evaluates the influence of Cu ions on biofilm formation on glass surface by the Oleidesulfovibrio alaskensis G20 (OA-G20) in a lactate-C medium supplemented with 30uM Cu ion. When exposed to media containing high levels of Cu ions, OA-G20 exhibited inhibited growth in its planktonic state. Whereas under the same experimental conditions, OA-G20 displayed enhanced biofilm formation on glass surfaces. To elucidate the molecular mechanisms underlying this adaptation, we performed a comparative proteomic analysis of OA-G20 stress-induced biofilms vs. control and their respective extracellular fractions.
Project description:Sulfate-reducing bacteria (SRB) are terminal members of any anaerobic food chain. For example, they critically influence the biogeochemical cycling of carbon, nitrogen, sulfur, and metals (natural environment) as well as the corrosion of civil infrastructure (built environment). The United States alone spends nearly $4 billion to address the biocorrosion challenges of SRB. It is important to analyze the genetic mechanisms of these organisms under environmental stresses. The current study uses transcriptome-wide marker gene panel mapping to decipher the stress mechanisms in SRB. This project contains 3 control samples and 6 test samples of RNA-seq data of Oleidesulfovibrio alaskensis strain G20, exposed to pristine copper and graphene-coated copper.
