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Dysregulation of cell migration by matrix metalloproteinases in geleophysic dysplasia

ABSTRACT: Dysregulation of cell migration by matrix metalloproteinases in geleophysic dysplasia

PROVIDER: PRJNA1240284 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32809059_1.fastq.gz	Fastqsanger.gz
	SRR32809059_2.fastq.gz	Fastqsanger.gz
	SRR32809060_1.fastq.gz	Fastqsanger.gz
	SRR32809060_2.fastq.gz	Fastqsanger.gz
	SRR32809061_1.fastq.gz	Fastqsanger.gz

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2023-03-07 | GSE223750 | GEO

Matrix Metalloproteinases After Surgery and/or Radiofrequency Ablation in Patients With Liver Metastases From Colorectal Cancer

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Regulation of matrix metalloproteinases (MMPs) expression and secretion by LIM and SH3 protein (LASP1)

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The gelatinases, matrix metalloproteinases 2 and 9, play individual roles in skeleton development

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Sensing of mycobacterial arabinogalactan by galectin-9 exacerbates mycobacterial infection by inducing matrix metalloproteinases

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Human apical-out nasal organoids reveal an essential role of matrix metalloproteinases in airway epithelial differentiation

Project description:Extracellular matrix (ECM) assembly/disassembly is a critical regulator for airway epithelial development and remodeling. Airway organoid is widely used in respiratory research, yet there is limited study to indicate the roles and mechanismsof ECM organization in epithelial growth and differentiation by using in vitro organoid system. Moreover, most of current Matrigel-based airway organoids are in basal-out orientation where accessing the apical surface is challenging. We present a novel human apical-out airway organoid using a biochemically defined hybrid hydrogel system. During human nasal epithelial progenitor cells (hNEPCs) differentiation, thegel gradually degraded, leading to the organoid apical surfaces facing outward. The expression and activity of ECM-degrading enzymes, matrix metalloproteinases (MMP7, MMP9, MMP10 and MMP13) increased during organoid differentiation,where inhibition of MMPs significantly suppressed the normal ciliation, resulting in increased goblet cell proportion. Moreover, a decrease of MMPs was found in goblet cell hyperplastic epithelium in inflammatory mucosa. This system reveals essential roles of epithelial-derived MMPs on epithelial cell fate determination, and provides an applicable platform enabling further study for ECM in regulating airway development in health and diseases.

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Human apical-out nasal organoids reveal an essential role of matrix metalloproteinases in airway epithelial differentiation

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