ABSTRACT: Metabolic coupling of aerobic and anaerobic carbon and nitrogen cycling in plateau riparian zones: asymmetric response in dry and rainy seasons
Project description:The rapid expansion of fast-growing plantations in subtropical regions is closely linked to dry-season irrigation and fertilization; however, improper practices often lead to soil acidification and reduced nutrient bioavailability. Phosphorus (P), one of the most critical elements for plantation tree growth, shows complex spatial distribution patterns in soil that are influenced by multiple factors, directly affecting plantation productivity. This study investigated the effects of long-term fertilization and dry-season irrigation on the vertical distribution of phosphorus in an 8-year-old subtropical Eucalyptus plantation. This study employed stratified sampling (0–30 cm topsoil, 30–60 cm subsoil, 60–90 cm substratum) during dry seasons, coupled with metagenomics, metabolomics, and environmental factor analysis, to reveal vertical phosphorus cycling patterns and multiomics regulatory networks. Key findings: (1) Fertilization and dry-season irrigation had a limited influence on labile phosphorus and the diversity of P-cycling microorganisms. The topsoil presented significantly greater P availability than did the subsoil, manifested as elevated acid phosphatase activity (ACP), significant enrichment of the tryptophan metabolic pathway, and greater microbial diversity. (2) pH and the C:P ratio represent critical factors of vertical stratification in soil P cycling. Under acidic conditions, topsoil microorganisms facilitate P release via diverse metabolic pathways, whereas oligotrophic constraints in the substratum limit enzymatic activities. (3) We believe that potential cross-stratum microbial functional coordination exists in acidic soil P cycling, with linkages to tryptophan metabolism and polyP synthesis/degradation. Our study provides theoretical multiomics insights for optimizing the management of soil P pools in subtropical plantations under fertilization and dry-season irrigation.
2025-12-09 | PXD070738 | Pride
Project description:Microbial of rhizosphere soil of teak intercropping Alpinia katsumadai Hayata in dry and rainy seasons
| PRJNA1125854 | ENA
Project description:Microbial characteristics of rhizosphere soil of teak intercropping Alpinia katsumadai Hayata in dry and rainy seasons
Project description:Anthropogenic nitrogen (N) deposition may affect soil organic carbon (SOC) decomposition, thus affecting the global terrestrial carbon (C) cycle. However, it remains unclear how the level of N deposition affects SOC decomposition by regulating microbial community composition and function, especially C-cycling functional genes structure. We investigated the effects of short-term N addition on soil microbial C-cycling functional gene composition, SOC-degrading enzyme activities, and CO2 emission in a 5-year field experiment established in an artificial Pinus tabulaeformis forest on the Loess Plateau, China.
Project description:Lung Cancer is the leading cause of cancer related deaths worldwide. Unfortunately, radiation resistance is a major problem facing lung cancer patients. Recently, we identified a group of long non-coding RNAs (lncRNAs) known as linc-SPRY3 RNAs, expressed from the Y-chromosome, that play a role in radiation sensitivity by decreasing tumor burden in vitro and in vivo after radiation. In this study, we found that the linc-SPRY3 RNAs are one large lncRNA that we named Radiation Induced Y-chromosome linked long non-coding RNA (lnc-RAINY). Through ATAC-Seq and Immunoprecipitation experiments, we show that lnc-RAINY interacts with DNA in a triple helix to change chromatin opening and gene expression. We also identified that lnc-RAINY regulates CDC6 and CDC25A expression affecting the induction of senescence, inhibition in cell migration, and cell cycle regulation. Furthermore, the administration of nanoparticle encapsulated lnc-RAINY into a lung cancer patient-derived xenograft model dramatically reduces tumor progression demonstrating its therapeutic potential.
Project description:Lung Cancer is the leading cause of cancer related deaths worldwide. Unfortunately, radiation resistance is a major problem facing lung cancer patients. Recently, we identified a group of long non-coding RNAs (lncRNAs) known as linc-SPRY3 RNAs, expressed from the Y-chromosome, that play a role in radiation sensitivity by decreasing tumor burden in vitro and in vivo after radiation. In this study, we found that the linc-SPRY3 RNAs are one large lncRNA that we named Radiation Induced Y-chromosome linked long non-coding RNA (lnc-RAINY). Through ATAC-Seq and Immunoprecipitation experiments, we show that lnc-RAINY interacts with DNA in a triple helix to change chromatin opening and gene expression. We also identified that lnc-RAINY regulates CDC6 and CDC25A expression affecting the induction of senescence, inhibition in cell migration, and cell cycle regulation. Furthermore, the administration of nanoparticle encapsulated lnc-RAINY into a lung cancer patient-derived xenograft model dramatically reduces tumor progression demonstrating its therapeutic potential.
2025-04-23 | GSE274484 | GEO
Project description:Microbial diversity in riparian zones affected by flooding
Project description:The dry season is a major challenge for Plasmodium falciparum parasites in many malaria endemic regions, where water availability limits mosquitoes to only part of the year. How P. falciparum bridges two transmission seasons months apart, without being cleared by the host or compromising host survival is poorly understood. Here we show that low levels of P. falciparum parasites persist in the blood of asymptomatic Malian individuals during the 5- to 6-month dry season, rarely causing symptoms and minimally affecting the host immune response. Parasites isolated during the dry season are transcriptionally distinct from those of subjects with febrile malaria in the transmission season, reflecting longer circulation within each replicative cycle, of parasitized erythrocytes without adhering to the vascular endothelium. Low parasite levels during the dry season are not due to impaired replication, but rather increased efficiency of splenic clearance of longer-circulating infected erythrocytes. We propose that P. falciparum virulence in areas of seasonal malaria transmission is regulated so that the parasite decreases its endothelial binding capacity, allowing increased splenic clearance and enabling several months of subclinical parasite persistence.
Project description:To identify the concurrent effects of multiple environmental factors on gene expression under natural climatic fluctuations in rice, we examined global gene expression patterns in two fields typical of the main modes of rice cultivation, over two seasons, each comprising 15 time-points in three different genotypes Two time-series of 15 time-points, one for each season (dry and wet) over two different fields
