Project description:We sought to better understand the mechanism of acid-induced cell death in Saccharomyces boulardii--a probiotic yeast routinely used to prevent and treat gastrointestinal disorders. To do this we generated microarray gene expression profiles of S. boulardii cells cultured in an acidic environment.
Project description:A comparative genomic hybridisation experiment using Affymetrix YG-S98 arrays to study the genetic background of S. Boulardii compared to S. Cerevisiae strain BY4743. Background: Saccharomyces boulardii, a yeast that was isolated from fruit in Indochina has been used as a remedy for diarrhoea since 1950, and is now a commercially available treatment throughout Europe, Africa and South America. Though initially classified as a separate species of Saccharomyces, recent publications have shown that the genome of S. boulardii is so similar to Saccharomyces cerevisiae that the two should be classified as conspecific. This raises the question of the distinguishing molecular and phenotypic characteristics present in S. boulardii that make it perform more effectively as a probiotic organism compared to other strains of S. cerevisiae. This investigation reports some of these characteristics including enhanced ability for pseudohyphal switching upon nitrogen limitation and increased resistance to acidic pH. However, these differences did not correlate with increased adherence to epithelial cells or transit through mouse gut. Pertinent characteristics of the S. boulardii genome such as trisomy of chromosome IX, altered copy number of a number of individual genes and sporulation deficiency have been revealed by comparative genome hybridisation using oligonucleotide-based microarrays coupled with a rigorous statistical analysis. The contributions of the different genomic and phenotypic features of S. boulardii to its probiotic nature are discussed.
Project description:Identifictaion of proteins from extracellular vesicles of probiotic yeasts Saccharomyces boulardii. This work was financially supported by the National Science Centre, Poland (grant number 2021/43/D/NZ6/01464).
Project description:In this study, the hypothesis that the different phenotypes exhibited by S. cerevisiae and S. boulardii lay on differential gene expression, based on promoter sequencing variability, is approached. To test this possibility, S. boulardii and S. cerevisiae were grown in intestinal like medium (ILM), optimized to enable the growth of both strains, and their transcriptome-wide expression patterns were evaluated through RNA-sequencing. mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NextSeq. The sequence reads that passed quality filters were analyzed with TopHat followed by HTSeq. RNA-seq data allowed to identify genes whose expression is differentially regulated in both organisms with a log2fold change ≥1 and p value <0.01. The RNA-Seq data was also used to refine the current S. boulardii annotation.
Project description:We sought to better understand the mechanism of acid-induced cell death in Saccharomyces boulardii--a probiotic yeast routinely used to prevent and treat gastrointestinal disorders. To do this we generated microarray gene expression profiles of S. boulardii cells cultured in an acidic environment. Two samples were analyzed. One replicate per array.
