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Epithelial Transcription Factor Elf3 mediates host immune responses to microbiota and protects against aerocystitis in zebrafish

ABSTRACT: Epithelial Transcription Factor Elf3 mediates host immune responses to microbiota and protects against aerocystitis in zebrafish

PROVIDER: PRJNA1245487 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32958950_1.fastq.gz	Fastqsanger.gz
	SRR32958950_2.fastq.gz	Fastqsanger.gz
	SRR32958951_1.fastq.gz	Fastqsanger.gz
	SRR32958951_2.fastq.gz	Fastqsanger.gz
	SRR32958952_1.fastq.gz	Fastqsanger.gz

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Epithelial Transcription Factor Elf3 mediates host immune responses to microbiota and protects against aerocystitis in zebrafish

Project description:We identified Elf3 as a conserved microbially responsive transcription factor and generated an elf3 mutant zebrafish allele to test whether it mediates host responses to microbes. We performed bulk RNA-sequencing of elf3 wildtype and mutant larvae reared without (germ-free) or with (conventionalized) the presence of microbes. We found that elf3 does mediate host-microbiota interactions in zebrafish and has an important role in the induction of many immune responsive genes.

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RNA-Seq and ChIP-Seq profiling of ELF3, an prion-like domain-containig in ELF3 that functions as a thermosensor in Arabidopsis.

Project description:Temperature is a major environmental variable governing plant growth and development. ELF3 contains a polyglutamine (polyQ) repeat 8–10, embedded within a predicted prion domain (PrD). We find the length of the polyQ repeat correlates with thermal responsiveness. Plants from hotter climates appear to have lost the PrD domain, and these versions of ELF3 are stable at high temperature and lack thermal responsiveness. ELF3 temperature sensitivity is also modulated by the levels of ELF4, indicating that ELF4 can stabilise ELF3 function. This RNA-Seq dataset provides evidence for the hypothetical ELF3 function of temperature sensing .

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Project description:Plants regulate their time to flowering by gathering information from the environment. Photoperiod and temperature are among the most important environmental variables. Suboptimal, but not near-freezing, temperatures regulate flowering through the thermosensory pathway, which overlaps with the autonomous pathway. Here we show that ambient temperature regulates flowering by two genetically distinguishable pathways, one that requires TFL1 and another that requires ELF3. The delay in flowering time observed at lower temperatures was partially suppressed in single elf3 and tfl1 mutants, whereas double elf3 tfl1 mutants were insensitive to temperature. tfl1 mutations abolished the temperature response in cryptochrome mutants that are deficient in photoperiod perception, but not in phyB mutants that have a constitutive photoperiodic response. Contrary to tfl1, elf3 mutations were able to suppress the temperature response in phyB mutants, but not in cryptochrome mutants. The gene expression profile revealed that the tfl1 and elf3 effects are due to the activation of different sets of genes and identified CCA1 and SOC1/AGL20 as being important cross talk points. Finally, genome-wide gene expression analysis strongly suggests a general and complementary role for ELF3 and TFL1 in temperature signalling. Three genotypes, WT (Columbia), elf3-7 and tfl1-1 mutants. Three biological replicates for each condition (genotype X temperature combination). RNA prepared independently for each sample.

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Anp32e protects against accumulation of H2A.Z at Sox motif containing promoters during zebrafish gastrulation

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