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Mus musculus

ABSTRACT: Genome-wide maps of RAP1 binding sites.

PROVIDER: PRJNA124659 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR038966.fastq.gz	Fastqsanger.gz
	SRR038967.fastq.gz	Fastqsanger.gz
	SRR038968.fastq.gz	Fastqsanger.gz
	SRR038969.fastq.gz	Fastqsanger.gz
	SRR038970.fastq.gz	Fastqsanger.gz

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Genome-wide maps of RAP1 binding sites.

Project description:By performing chromatin immunoprecipitation coupled with ultra-high-throughput sequencing (ChIP-seq), we find that RAP1 binds to telomeres as well as to extra-telomeric sites through the (TTAGGG)2 consensus motif. Extra-telomeric RAP1 binding sites are particularly abundant at subtelomeric regions, and this is in agreement with preferential deregulation of subtelomeric genes in Rap1-deficient cells. Significantly, more than 70% of extratelomeric RAP1 binding sites are located in the vicinity of known genes and about 40% of the genes deregulated in Rap1-null cells contain binding sites for RAP1, suggesting a role of RAP1 in transcriptional control. Examination of RAP1 binding by CHIP-seq in two independent wild-type mefs using as negative control two independent RAP1-deleted mefs

2010-07-10 | E-GEOD-20867 | biostudies-arrayexpress

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