Project description:Male and female 2- and 3-years old brown bear from the region of Tackasen (Sweden) were captured in summer and winter. Muscle biopsies from vastus lateralis were collected in february (hibernation state) and june (active period). Total RNA were extracted from muscle tissue and full transcriptome analysis (RNA Seq) were performed. Statistical analysis were performed to winter versus summer comparison from matched paired samples
Project description:Transcriptomic analysis of the brown bear (Ursus arctos) tissues during both active and inactive phases has unveiled significant differential gene expression, with thousands of genes exhibiting altered activity in all studied tissues (muscle, liver, adipose...). Nevertheless, transcriptional analyses of hibernating animal tissues are limited to organs that are important in metabolism. No information is available about the variations of gene expression in tissues that may have a lower impact on metabolism, like the skin, which is mainly a support tissue and a mechanical barrier against the external environment. That experiment brings information about the gene expression variations in the bear skin during hibernation.