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Beauveria bassiana

ABSTRACT: Transcriptome of the Beauveria bassiana (Bb0062) BbOsrR2/BbOsrR3/BbClp1 Disruption Mutant under Normal and Cell Wall Stress (CR/CFW) Conditions

PROVIDER: PRJNA1248725 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33225540_1.fastq.gz	Fastqsanger.gz
	SRR33225540_2.fastq.gz	Fastqsanger.gz
	SRR33225541_1.fastq.gz	Fastqsanger.gz
	SRR33225541_2.fastq.gz	Fastqsanger.gz
	SRR33225542_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of LaeA and LaeB deletion strains in fungus Beauveria bassiana

Project description:Transcriptomic analysis of LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana Examination of differential gene expressions by Beauveria bassiana wild type, LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana

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Profound effect of histone methyltransferase (DIM5) on genomic expression of Beauveria bassiana

Project description:Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of dim5 disruption and wild-type strains grown under normal culture conditions and and subjected to deep sequencing on Illumina Novaseq 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10915 genes annotated or not annotated. Among those, 1201 genes (genomic 11.59%) were dysregulated in the absence of dim5, including 489 down-regulated and 712 up-regulated. Conclusions: DIM5 has profound impact on the genomic expression of Beauveria bassiana.

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Profound effect of a small cysteine-free protein (pspA) on genomic expression of Beauveria bassiana

Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by pspA, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of pspA disruption and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10430 genes annotated or not annotated. Among those, 1818 genes (genomic 17.54%) were dysregulated in the absence of pspA, including 806 down-regulated and 1012 up-regulated. Conclusions: pspA lacking any predictable function domain has profound impact on the genomic expression of Beauveria bassiana.

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Project description:This project mainly focuses on the proteomics analysis of Beauveria bassiana ARSEF 2860 under various cultural conditions. Through comparative proteomics analysis between wild-type strains and gene-disruption strains, we aim to get a global view of the whole proteins level for a better understanding of the mechanism of biocontrol potential in Beauveria bassiana ARSEF 2860.

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