Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1250800 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33144830_1.fastq.gz | Fastqsanger.gz | |||
| SRR33144830_2.fastq.gz | Fastqsanger.gz | |||
| SRR33144831_1.fastq.gz | Fastqsanger.gz | |||
| SRR33144831_2.fastq.gz | Fastqsanger.gz | |||
| SRR33144832_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 120 |
Similar Datasets
Project description:To unravel molecular targets involved in glycopeptide resistance, three isogenic strains of Staphylococcus aureus with different susceptibility levels to vancomycin or teicoplanin were subjected to whole-genome microarray-based transcription and quantitative proteomic profiling. Quantitative proteomics performed on membrane extracts showed exquisite inter-experimental reproducibility permitting the identification and relative quantification of >30% of the predicted S. aureus proteome. In the absence of antibiotic selection pressure, comparison of stable resistant and susceptible strains revealed 94 differentially expressed genes and 178 proteins. As expected, only partial correlation was obtained between transcriptomic and proteomic results during stationary-phase. Application of massively parallel methods identified one third of the complete proteome, a majority of which was only predicted based on genome sequencing, but never identified to date. Several over‑expressed genes represent previously reported targets, while series of genes and proteins possibly involved in the glycopeptide resistance mechanism were discovered here, including regulators, global regulator attenuator, hyper‑mutability factor or hypothetical proteins. Gene expression of these markers was confirmed in a collection of genetically unrelated strains showing altered susceptibility to glycopeptides. Our proteome and transcriptome analyses have been performed during stationary‑phase of growth on isogenic strains showing susceptibility or intermediate level of resistance against glycopeptides. Altered susceptibility had emerged spontaneously after infection with a sensitive parental strain, thus not selected in vitro. This combined analysis allows the identification of hundreds of proteins considered, so far as hypothetical protein. In addition, this study provides not only a global picture of transcription and expression adaptations during a complex antibiotic resistance mechanism but also unravels potential drug targets or markers that are constitutively expressed by resistant strains regardless of their genetic background, amenable to be used as diagnostic targets. Keywords: Molecular markers, antibiotic resistance, glycopeptides, growth-phase
2007-03-09 | GSE5188 | GEO
Project description:We investigate the differences in total gene expression between P.aeruginosa PAO1 (wild-type) and mexZ isogenic mutant. Inactivation of mexZ is known to lead to the overexpress of mexX and mexY genes and being involved in intrinsic resistance antibiotic, in particular to aminoglycosides. Cells were grown in a rich medium without any selective pressure (shaking at 37C) and cells were harvested during mid-log phase at OD600 0.5.
2018-08-21 | E-MTAB-5820 | biostudies-arrayexpress
Project description:We investigate the differences in total gene expression between P.aeruginosa PAO1 (wild-type) and ΔersA isogenic mutant. ErsA is a small RNA involved in several P. aeruginosa phenotypes, including biofilm formation and antibiotic resistance. Cells were grown in a rich medium (BHI, Brain Heart Infusion) without any selective pressure (shaking at 37°C) and cells were harvested during stationary phase at OD600 2.7.
2018-02-05 | E-MTAB-6247 | biostudies-arrayexpress
Project description:Pseudomonas aeruginosa infections can be virtually impossible to eradicate and the evolution of resistance during antibiotic therapy is a significant concern. In this study, we use DNA microarrays to characterize the global transcriptional response of P. aeruginosa to clinical-like doses of the antibiotic ciprofloxacin and also to determine the component that is regulated by LexA cleavage and the SOS response. We find that genes involved in virtually every facet of metabolism are down-regulated in response to ciprofloxacin. The LexA-controlled SOS regulon identified by microarray analysis includes only fifteen genes, but does include several genes that encode proteins involved in recombination and replication, including two inducible polymerases known to play a role in mutation and the evolution of antibiotic resistance in other organisms. The data suggests that the inhibition of LexA cleavage during therapy might help combat this pathogen by decreasing its ability to adapt and evolve resistance. Keywords: Time course of response of P. aeruginosa to the antibiotic ciprofloxacin
2006-08-04 | GSE5443 | GEO