Ontology highlight
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PROVIDER: PRJNA1250823 | ENA |
REPOSITORIES: ENA
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Project description:Background: Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives: In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods: Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (Tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results: In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions: We screened 1645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.
2022-05-19 | PXD031331 | Pride
Project description:Heat stress is a major challenge for crop production. During the reproduction stage, pollen development is the most sensitive process to abnormal environmental conditions. In this project, we have developed a heat treatment system for tomato where plants produce a significantly lower number of pollens and the germination rate of those pollens was also very low. The size of the flower buds and pollen developmental stages were examined under confocal microscope. Pollen cells at different developmental stages were collected from flower buds at corresponding sizes using LCM. The single cell population proteomics analysis was used to identify proteome changes in a distinct group of cells. This information was used to determine the function of proteins that are associated with heat stress on cellular processes/functions during pollen development.
2022-02-23 | PXD010218 | Pride
Project description:- Pollen tube growth is important process for successful double fertilization, which is critical for grain yield in crop plants. Despite much progress in identification of rapid alkalization factors (RALFs) which serve as ligand for signaling transduction during fertilization in Arabidopsis, there is no functional study of RALF in mono-cotyledon plant. - We functionally characterized two pollen specific RALF in rice (Oryza sativa) using multiple CRISPR/Cas9 induced loss-of-function mutants, peptide treatment, expression analyses, tag reporter lines. - OsRALF17 is specifically expressed in pollen and pollen tube as the strongest level among 41 RALF members in rice. Exogenously applied OsRALF17 inhibits pollen tube germination and elongation at high concentration, but enhances tube elongation at low concentration, indicating the regulation of growth balance. Double mutant of OsRALF17 with OsRALF19 exhibit almost male sterile, with defect on pollen germination and tube elongation. - Our study revealed that functionally-redundant OsRALF17 and 19 peptides binds to the OsMTD2, CrRLK1L family member, and transmits ROS signal for pollen tube germination and integrity maintenance in rice. We provide new insights into the role of RALF and expanding our understanding of the biological role of RALF in regulating rice fertilization.
2023-09-21 | PXD037597 | Pride
Project description:Artificial mutations in nuclear gene encoding mitochondrial RNA polymerase restore pollen fertility in cytoplasmic male sterile tomato
| PRJDB20274 | ENA
Project description:Artificial mutations in nuclear gene encoding mitochondrial RNA polymerase restore pollen fertility in cytoplasmic male sterile tomato
| PRJDB20280 | ENA
Project description:We employed a microarray-based transcriptomic status comparison of rice cytoplasmic male sterile (CMS) lines in order to categorize the nuclear gene expressions upon cytoplasmic substitution. In anthers at the uninucleate and bicellular pollen stages, we found that 8,199 genes significantly changed their expression in at least one of the CMS lines. We categorized the genes into 100 clusters by k-means clustering, and common obvious expression patterns were observed in W11, LD and BT.
2010-08-22 | GSE18057 | GEO
Project description:Our results show that ICE1 controls plant male fertility via impacting anther dehydration. The loss-of-function mutation in ICE1 gene in Arabidopsis caused anther indehiscence and decreased pollen viability as well as germination rate. Further analysis revealed that the anthers in the mutant of ICE1 (ice1-2) had the structure of stomium, though the epidermis did not shrink to dehisce. The anther indehiscence and influenced pollen viability as well as germination in ice1-2 were due to abnormal anther dehydration, for most of anthers dehisced with drought treatment and pollen grains from those dehydrated anthers had similar viability and germination rates compared with wild type. Accordingly, the sterility of ice1-2 could be rescued by ambient dehydration treatments. Likewise, the stomatal differentiation of ice1-2 anther epidermis was disrupted in a different manner compared with that in leaves. ICE1 specifically bound to MYC-recognition elements in the promoter of FAMA, a key regulator of guard cell differentiation, to activate FAMA expression. Transcriptome profiling in the anther tissues further exhibited ICE1-modulated genes associated with water transport and ion exchange in the anther. Together, this work reveals the key role of ICE1 in male fertility control and establishes a regulatory network mediated by ICE1 for stomata development and water movement in the anther.
2018-09-13 | GSE107260 | GEO
Project description:Upon germination, pollen forms a tube that elongates dramatically through female tissues in order to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We used Affymetrix Arabidopsis ATH1 Genome Arrays covering more than 80% of the Arabidopsis genome to compare transcriptomes of cell-sorted, hydrated pollen grains with those of flowers, leaves, seedlings and siliques (all samples with duplicates). This comparison revealed that pollen expresses a reduced set of genes with increased proportions of enriched and selectively-expressed transcripts. Relative gene ontology (GO) category representations in pollen and vegetative tissues revealed a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Relative gene ontology (GO) category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Relative gene ontology (GO) category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome towards signaling, vesicle transport and cytoskeleton, suggestive of a commitment towards germination and tube growth. Gene family and pathway analysis allowed formulation of novel hypotheses for the role of non-classical MADS-box genes, small RNA pathways and cell cycle components in pollen.
2005-06-01 | E-MEXP-285 | biostudies-arrayexpress
Project description:Pollen grains are coated with a pollen wall which protects the microspore from various biotic and abiotic stresses, as well as facilitates male-female interaction. During pollen wall development, many compounds are transported from the tapetum to the developing pollen wall via tapetum-specific membrane transporters, and male sterility is often observed when membrane transporter’s function or localization is altered. Here, we show that mutating AP1/2β1 and AP1/2β2, two homologous genes of AP1/2β which encodes a large subunit shared by adaptor protein 1 (AP-1) and adaptor protein 2 (AP-2) complexes in Arabidopsis, impair pollen exine formation and cause reduced pollen germination rate and slowed pollen tube growth. However, the shared theory of AP1/2β is not proved in Arabidopsis.
2022-10-14 | PXD031514 | Pride
Project description:The RNA-seq was used to identify differentially regulated miRNAs between a male sterile and wild type tomato during anther development.
2015-10-01 | GSE65788 | GEO