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ABSTRACT: plant material leaf Genome

PROVIDER: PRJNA1252368 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33208358_1.fastq.gz	Fastqsanger.gz
	SRR33208358_2.fastq.gz	Fastqsanger.gz

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Project description:plant material leaf Genome

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Systems genetics reveals a transcriptional network associated with susceptibility in the maize-gray leaf spot pathosystem

Project description:Background: Maize plants developed typical gray leaf spot disease (GLS) symptoms initiating at the lower leaves and progressing to upper leaves through the season. Leaf material was collected at 77 days after planting, at which stage there were a large number of GLS disease necrotic lesions on lower leaves (8% surface area on average determined by digital image analysis), but very few lesions and only at chlorotic stage on leaves above the ear (average of 0.2% lesion surface area). Method:To collect material that reflected a difference between C.zeina infected B73 leaves and control B73 leaf material, samples were collected from two lower GLS infected leaves (second and third leaf internode below ear) , and two upper leaves with minimal GLS symptoms (second and third internode above ear), respectively. The two lower leaves from each plant were pooled prior to RNA extraction, and the two upper leaves from each plant were pooled prior to RNA extraction. Upper and lower leaf samples from three maize B73 plants were subjected to RNA sequencing individually. The three maize plants were selected randomly as one plant per row from three rows of ten B73 plants each. Result: A systems genetics strategy revealed regions on the maize genome underlying co-expression of genes in susceptible and resistance responses, including a set of 100 genes common to the susceptible response of sub-tropical and temperate maize.

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Transcription profiling of Arabidopsis mature leaf 6 of ron2-1 EMS mutant vs wild type Ler

Project description:Goal:identify the genes regulated by RON2 involved in the delay of floral transitional and those associated with leaf development. The gene expression profile of mature leaf 6 of ron2-1 EMS mutant and the wild-type Ler are compared. The plant material for this experiment was grown in LEPSE-INRA Montpellier, and the microarrays at the MAF-VIB Leuven with PSB-VIB Ghent.

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Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense: plp2 Botrytis cinerea

Project description:af13_plp2 - plp2 botrytis cinerea 2 - Effects of deregulation of a lipid acyl hydrolase gene (PLP2, At2g26560) on global transcriptome upon infection by Botrytis cinerea. This deregulation affects resistance levels against fungal and bacterial pathogens, likely by perturbing the biosynthesis of oxylipins. Oxylipins are fatty acid-derived compounds (example:jasmonic acid) with diverse signaling or antimicrobial properties. - 5000 spores of Botrytis were pipetted on 4 infection sites per ault leaf. Leaf material was harvested at 0 and 2 days later. 3 plant genotypes were used (Col-0 ecotype): siPLP2 (RNAi-silenced),pBIN (empty pBIN-transformed),PLP2OE (PLP2-overexpressors).

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plp2 botrytis cinerea 2-Functional analysis of lipid acyl hydrolases in Arabidopsis antimicrobial defense

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