Project description:To identify candidate downstream mRNA target(s) for hsa-miR-616 Affymetrix Human Genome U133 Plus GeneChip 2.0 miR-616 or empty vector control was stably overexpressed in LNCaP prostate cells using lentivirus
Project description:Proteome data derive from three different types of cells （uninfected gADSCs, gADSCs infected with the empty lentiviral vector, and gADSCs infected with the miR-204-5p lentiviral vector).
Project description:To determine genes regulated by HNF4G, we retrovirally infected LNCaP cells in triplicates to express HNF4G and empty vector as control. cDNA for HNF4G in pDONR201 vector was obtained from Harvard medical school Plasmid database (ID:HsCD00022314) and was cloned into an murine stem cell virus (MSCV)-based retroviral vector with puromycin selection marker (Addgene) using Gateway technology.
Project description:Transcriptional profiling of human 786-O cells comparing total RNA from cells transfected with the pCEP4 vector containing a Bcl-xAS lncRNA construct vs. control empty pCEP4 vector-transfected cells. Goal was to determine the effects of Bcl-xAS overexpression on global 786-O cells gene expression.
