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ABSTRACT: Transcriptomic changes during symptomatic and symptom recovery phase of Nepovirus lycopersici (tomato ringspot virus) infection

PROVIDER: PRJNA1253310 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33227821_1.fastq.gz	Fastqsanger.gz
	SRR33227821_2.fastq.gz	Fastqsanger.gz
	SRR33227822.fastq.gz	Fastqsanger.gz
	SRR33227823_1.fastq.gz	Fastqsanger.gz
	SRR33227823_2.fastq.gz	Fastqsanger.gz

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Project description:Field-grown tubers of potato were examined for infection by Tobacco rattle virus and consequent production of corky ringspot or spraing symptoms. A microarray study identified tuber genes that are differentially expressed in response to TRV infection and to spraing production, showing that hypersensitive response (HR) pathways are activated in spraing-symptomatic tubers. This was confirmed by quantitative RT-PCR (Q-RT-PCR) of a selected group of HR-related genes and by histochemical staining of excised tuber tissue with spraing symptoms. Q-RT-PCR of TRV in different areas of the same tuber slice showed that non-symptomatic areas contained higher levels of virus than did spraing-symptomatic areas. This suggests that spraing formation is associated with an active plant defence that reduces the level of virus in the infected tuber. Expression of two plant defence genes was similarly upregulated in spraing-symptomatic tubers that were infected with another virus, Potato mop-top-virus, suggesting that spraing is a generalised response to virus infection of tubers.

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