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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [degradome]

ABSTRACT: RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [degradome]

PROVIDER: PRJNA1254188 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR33253263.fastq.gz	Fastqsanger.gz
	SRR33253264.fastq.gz	Fastqsanger.gz
	SRR33253265.fastq.gz	Fastqsanger.gz
	SRR33253266.fastq.gz	Fastqsanger.gz
	SRR33253267.fastq.gz	Fastqsanger.gz

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [degradome]

Project description:Identification of genes involved in the regulation of taproot and lateral root growth in Quercus robur seedlings under drought stress and well-watered conditions. Genes involved in the regulation of taproot and lateral root growth in Quercus robur seedlings were identified using RNA-seq, miRNA-seq, and degradome-seq. The analysis focused on the gene expression, miRNA regulation, and mRNA degradation profiles of taproots and lateral roots under both drought stress and well-watered conditions. Key genes and their regulatory miRNAs were identified, along with the role of mRNA degradation pathways in response to stress, providing insights into the molecular mechanisms controlling root growth and development in oak seedlings under varying water availability.

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [miRNA-seq]

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [RNA-seq]

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [RNA-seq]

Project description:RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [RNA-seq]

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [miRNA-seq]

Project description:RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [miRNA-seq]

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RNA-seq analysis of different parts of Quercus robur seedling root

Project description:As an adaptive response to the environment, oaks have evolved long taproots that increase their ability to acquire water. Taproots penetrate deeper layers of soil and send out absorptive roots that can then uptake water from these layers. This ability enables taproots to supply trees with water better. Unfortunately, the applied agrotechnical procedures during seedlings production in container nurseries damage the oaks' taproot, leading to changes in the root system structure. That changes may make the seedlings more responsive to chronic or periodic episodes of severe drought. In turn, seedlings that containers do not restrict roots growth, i.e. grown in rhizotron, may elongate because they are not subjected to air-pruning. Despite their significant role, little is known about the internal factors (specific genes) and their interactions that regulate taproot elongation in oaks seedlings. Thus, our study aimed to determine the potential genes regulating growth, cessation and physiology of taproot and check whether there is a difference in the expression level of the genes involved in root development. For this purpose, we performed next-generation sequencing (NGS), taproots and lateral roots, which allowed us to obtain a complete picture of the transcriptomes. Our findings of taproot growth regulations can be used to improve trees production in forest nurseries.

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