Parallel gene expression changes in ventral midbrain dopamine and GABA neurons during normal aging
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ABSTRACT:
PROVIDER: PRJNA1254295 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33263057.fastq.gz | Fastqsanger.gz | |||
| SRR33263057_1.fastq.gz | Fastqsanger.gz | |||
| SRR33263057_2.fastq.gz | Fastqsanger.gz | |||
| SRR33263058.fastq.gz | Fastqsanger.gz | |||
| SRR33263058_1.fastq.gz | Fastqsanger.gz |
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Project description:The circadian nature of mood and its dysfunction in affective disorders is well recognized, but the underlying molecular mechanisms are still unclear. We showed that the circadian nuclear receptor REV-ERBa, which is associated with bipolar disorder, impacts midbrain dopamine production and mood-related behavior in mice. Genetic deletion of the Rev-erba gene or pharmacological inhibition of REV-ERBa activity in the ventral midbrain induced mania-like behavior in association with a central hyperdopaminergic state. We used microarrays to identify differentially expressed genes in the ventral midbrains of wild-type (WT) and Rev-erba knock-out (RKO) mice. Male RKO and WT mice (10-15 weeks of age) were maintained in a C57BL/6J background. Mice were housed in temperature-controlled (22-23ºC) quarters under a 12-h light-dark (LD) photoperiod (lights on at 8:00 a.m.). After entrainment for >10 days under LD conditions, mice were kept in constant darkness (DD) for 2 days starting at lights-off time. On the third day, mice were sacrificed at indicated time points by cervical dislocation.
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