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Homo sapiens strain:human embryonic kidney cells

ABSTRACT: HEK293 Transcriptome during rAAV production (HEK293 cells from Mass Biologics in BalanCD HEK293 Medium)

PROVIDER: PRJNA1254762 | ENA |

REPOSITORIES: ENA

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AAV production system proteomics

Project description:We performed absolute quantification (AQUA) of viral proteins by targeted quantitative proteomics and conducted tandem mass tag (TMT)-based proteomics on the three rAAV and wtAAV production systems to identify potential factors limiting rAAV productivity in HEK293 cells

2024-07-03 | PXD050484 | Pride

Expression data from human embryonic kidney cells (HEK293) cultivated in high and low glucose

2009-04-08 | GSE15575 | GEO

Project description:HEK293 Transcriptome during rAAV production (ATCC HEK293 cells adapted in AMBIC media)

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Expression data from human embryonic kidney cells (HEK293) cultivated in high and low glucose

Project description:Gene expression profiling of human embryonic kidney (HEK293) cells was performed to determine the effect of high and low glucose on gene expression. Microarrays were used to identify distinct classes of genes up-regulated in HEK293 cells during cultivation for 7 days in medium with physiological (low) glucose compared to high glucose. Human embryonic kidney cells (HEK293) were cultivated for 7 days in commercially available DMEM (supplemented with 10 % FCS) containing high glucose (450 mg/dl) or low glucose (100 mg/dl). Cells were harvested, total RNA was extracted and microarray gene expression profiling was performed to compare differential gene expression between low and high glucose conditions. Two biological replicates for each condition were made (high glucose: HEK-high-gluc-1 and HEK-high-gluc-2; and low glucose: HEK-low-gluc-1 and HEK-low-gluc-2).

2009-04-08 | E-GEOD-15575 | biostudies-arrayexpress

Transcriptomics-informed pharmacology identifies epigenetic and cell cycle regulators as enhancers of AAV production

Project description:Recombinant adeno-associated virus (rAAV) is a widely used viral vector for gene therapy. Despite its clinical efficacy, the manufacturing of rAAV faces challenges in productivity and quality, leading to limited availability. To address the growing demand, next-generation process development should be informed by a mechanistic understanding of the cellular response to rAAV. In this study, we performed transcriptomic analysis of 5 cell lines with variable capacities for rAAV production. Using an intersectional approach, we assessed the transcriptional response to rAAV production and compared transcriptional profiles between high and baseline producers to identify possible targets for enhancing production. Modulation of cell cycle and nucleosome components suggested a reduction of proliferative capacity and a shift toward DNA replication to support rAAV production. During rAAV production, we observed upregulation of several core functions including transcription, stress response, and Golgi and endoplasmic reticulum organization. Conversely, inhibitors of DNA-binding proteins and mitochondrial components were consistently downregulated during rAAV production. We next performed a drug connectivity analysis of these results and identified 5 classes of drugs predicted to enhance rAAV production. Validation studies confirmed the efficacy of HDAC and microtubule inhibitors. Our data uncover novel and previously identified pathways that may enhance rAAV productivity, potentially enabling a path to engineer improved processes and cell lines for higher yields and better quality rAAV production.

2024-08-29 | GSE269485 | GEO

Project description:Single-cell transcriptomics of transfection-based rAAV production in HEK293 cells

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Time dependent salicylate treatment effect on human embryonic kidney cell line

2014-10-24 | GSE62655 | GEO

Time dependent salicylate treatment effect on human embryonic kidney cell line

Project description:Analysis of HEK293 cells treated with salicylate. 50 mM final concentration of sodium salicylate was treated to HEK293 cells, and their mRNA expression perturbation was observed time-dependent manner. Total RNA obtained from HEK293 cells was compared after salicylate treatment (30 min and 60 min).

2014-10-24 | E-GEOD-62655 | biostudies-arrayexpress

Multiplexed quantitative proteomic analysis of Human Embryonic Kidney 293 (HEK293)

Project description:Study of the changes in the HEK293 proteome when growing without transfection, when transfected with an empty plasmid and when transfected with Gagcoding gene to produce Gag VLPs,

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Gene expression profiles of wild-type and PERK-deficient cells during activation of OXPHOS

Project description:Intracellular ATP is mainly produced by the glycolysis pathway in culture cells incubated with high-glucose medium, whereas ATP production is switched to the OXPHOS pathway by culturing with the no-glucose medium. To elucidate the potential function of PERK during activation of OXPHOS pathway, we perfomed RNA-sequencing analayais using wild-type and PERK-deficient HEK293 cells.

2020-02-03 | GSE113572 | GEO

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