Project description:Understanding the regulatory roles of small RNAs (sRNAs) in Mycobacterium marinum is crucial for elucidating its pathogenesis. Here, we present transcriptome profiles of M. marinum strains with deletions and completions of sRNA B11. Through RNA sequencing analysis, we identified significant alterations in gene expression patterns between the B11-deleted and completed strains.
Project description:RNA-seq analysis of Pseudomonas sp OST1909 exposed to various preparations of naphthenic acids samples led to the identiifcation of many NA-induced genes.
Project description:WT M. abscessus ATCC_19977 was used to construct a targeted deletion mutant lacking the sRNA B11 and a complemented version of the mutant with ectopic expression of B11 from its native promoter at the L5 site. The deletion mutant was also complemented with versions of B11 that lacked a single C from the first loop or had an extra C in the second loop. All strains were grown to log phase and the extracted RNA was used to perform expression profiling by RNAseq.
Project description:The whole proteome analysis of the Pseudomonas sp. FIP_A4 strain in presence and absence of fipronil was conducted to evaluate the differentially expressed enzymes that can play role in fipronil degradation.
