Project description:Brassinosteroids (BRs) are a group of plant steroid hormones that play crucial roles various of growth and developmental processes. Biological function and signal transduction pathway of BR has been well characterized in model plants like Arabidopsis and rice. However, their biological roles are still unclear in tree species, especially in an important non-timber plant moso bamboo, which has great ecological and economic value and distinguish fast-growth feature. Here we reported that reduce endogenous brassinosteroid by biosynthesis inhibitor propiconazole reduced both root and shoot growth in seedling stage. Exogenous BR application promoted shoot bract elongation and inclination of lamina joint and bract. Genome-wide transcriptome analysis were performed to identify hundreds of differential expressed genes by BR and propiconazole treatment in shoot and root parts of bamboo seedling, respectively. GO analysis revealed that BR regulates cell wall related genes, hydrogen peroxide catabolic genes and auxin related genes to promote bamboo root development and elongation. Our study identified BR response genes and provides a comprehensive resource for molecular mechanism research of bamboo growth.
Project description:Moso bamboo (Phyllostachys edulis) is one of the fast-growing plant species and has high comprehensive utilization value. However, how Moso bamboo realize the transition from initial growth of winter shoot to fast growth of spring shoot is still unknown. Large increase of biomass in spring shoot suggests that alterations in bioenergetic processes may contribute to fast growth initiation. In this study, we successfully isolated mitochondria from winter shoots and spring shoots of Moso bamboo, and performed a total and mitochondrial transcriptomic and proteomic analysis using RNA sequencing and Label-free quantitative proteomics technology. The main objective of the study was to augment the genomic and proteomic data available for Moso bamboo, identify key genes/proteins involved in energy metabolism, and systematically understand the energy metabolism mechanism of shoots growth during the transitory stage from initial growth to rapid growth.