Project description:Screening of miRNA mimics resulted in differential motility capacity. To discover novel miRNA motility regulators, gene expression of breast cancer cells transfected with miRNA mimics were compared.
Project description:HMDMs were transfected with miRNA mimics negative control or miRNA-204-3p mimics for 24 hours, and then were sequenced to determine gene expression profiles.
Project description:We examined the influence of chemical modifications patterns in a set of miR-200c mimics, assessing the regulation of the whole transcriptome in A549 cells. We examined 37 mimics and provided an initial set of rules for designing miRNA mimics with potency and selectivity similar to an unmodified miRNA duplex.
Project description:Experiment 2 - MiRNA mimics have a length and passenger strand specific effect Wildtype and mutant variants of miR-155 and negative control miRNA mimics were transfected to address the phenotype
Project description:Experiment 2 - MiRNA mimics have a length and passenger strand specific effect Wildtype and mutant variants of miR-155 and negative control miRNA mimics were transfected to address the phenotype Cells were transfected with mimics and samples were collected in duplicate (except 23mer miR-155 wild type mimic and 23mer negative control mimic)
Project description:We examined the influence of chemical modifications patterns in a set of miR-200c, miR-155 and miR-34a mimics, assessing the regulation of the whole transcriptome in A549 cells. We examined the mimics and provided an initial set of rules for designing miRNA mimics with potency and selectivity similar to an unmodified miRNA duplex.
Project description:We compared histone modification states between miRNA-204-3p and mimics negative control transfected HMDMs through ChIP. sequencing
Project description:Analysis of gene expression change in U2OS cells expression synthetic miR-542-3p mimics. Forty-eight hours after transfection with negative miRNA mimics or miR-542-3p mimics, U2OS cells were subjected to RNA isolation.
Project description:Biotin labelled miR-206 mimics ('06") and miR-Control mimics ("NC") were used to indentify putative miRNA transcript targets in FAPs form skeletal muscle