Flaveria bidentis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1259992 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR33595200_1.fastq.gz | Fastqsanger.gz | |||
| SRR33595200_2.fastq.gz | Fastqsanger.gz | |||
| SRR33595201_1.fastq.gz | Fastqsanger.gz | |||
| SRR33595201_2.fastq.gz | Fastqsanger.gz | |||
| SRR33595202_1.fastq.gz | Fastqsanger.gz |
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Project description:Flaveria bidentis Transcriptome or Gene expression
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Project description:Flaveria bidentis Transcriptome or Gene expression
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Project description:The genus Flaveria has been extensively used as a model to study the evolution of C4 photosynthesis as it contains both C3 and C4 species as well as a number of species that exhibit intermediate types of photosynthesis. The current phylogenetic tree of the Flaveria genus contains 21 of the 23 known Flaveria species and has been constructed using a combination of morphologicial data and three non-coding DNA sequences (nuclear encoded ETS, ITS and chloroplast encoded trnl-F). However, recent studies have suggested that phylogenetic trees inferred using a small number of molecular sequences may often be incorrect. Moreover, studies in other genera have often shown substantial differences between trees inferred using morphological data and those using molecular sequence. To provide new insight into the phylogeny of the genus Flaveria we utilize RNA-Seq data to construct a multi-gene concatenated phylogenetic tree of 17 Flaveria species. Furthermore, we use this new data to identify 14 C4 specific non-synonymous mutation sites, 12 of which (86%) can be independently verified by public sequence data. We propose that the data collection method provided in this study can be used as a generic method for facilitating phylogenetic tree reconstruction in the absence of reference genomes for the target species. 18 Flaveria sample including 11 species are sequenced, other three samples were also sequenced as out-group. In all, 21 samples.
2015-01-08 | E-GEOD-54339 | biostudies-arrayexpress
Project description:The genus Flaveria has been extensively used as a model to study the evolution of C4 photosynthesis as it contains both C3 and C4 species as well as a number of species that exhibit intermediate types of photosynthesis. The current phylogenetic tree of the Flaveria genus contains 21 of the 23 known Flaveria species and has been constructed using a combination of morphologicial data and three non-coding DNA sequences (nuclear encoded ETS, ITS and chloroplast encoded trnl-F). However, recent studies have suggested that phylogenetic trees inferred using a small number of molecular sequences may often be incorrect. Moreover, studies in other genera have often shown substantial differences between trees inferred using morphological data and those using molecular sequence. To provide new insight into the phylogeny of the genus Flaveria we utilize RNA-Seq data to construct a multi-gene concatenated phylogenetic tree of 17 Flaveria species. Furthermore, we use this new data to identify 14 C4 specific non-synonymous mutation sites, 12 of which (86%) can be independently verified by public sequence data. We propose that the data collection method provided in this study can be used as a generic method for facilitating phylogenetic tree reconstruction in the absence of reference genomes for the target species.
2015-01-08 | GSE54339 | GEO
Project description:C4 photosynthesis overpasses C3 by a higher photosynthetic rate, along with a higher water and nitrogen use efficiency. The establishment of C4 photosynthesis requires modification of ancestral C3 in both anatomical and metabolic aspects, both are involved in many functional pathways which have not been fully demonstrated. This study aims to illustrate overall functional changes along the evolution of C4 photosynthesis, with a purpose to gain an overall view of what are required for C4 photosynthesis. To achieve this, this study assembled chromosome scale of genome sequencing of five Flaveria species, representing gradual evolutionary stages of C4 photosynthesis from C3 to C3-C4 and to C4. Here, result showed that the genome size gradually increases from C3 to intermediate to C4 species, which ranges from 0.5 G to 1.87 G. The enlarged genomes are mainly from transposable elements, with the number of protein coding gene are comparable along evolution. The copy of C4 version CA1 and PEPC1, as well as PEPC-k gained extra one, two and five copies in C4 species F. trinervia as a result of tandem duplication, which plays a positive role for the emergency of C4 photosynthesis. Besides, the evolutionary pattern of C4 gene on transcript abundance are consistent with that on protein abundance, suggesting transcriptional regulation is a key factor for establishing C4 photosynthesis.
2025-01-21 | PXD024720 | Pride