Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human immunodeficiency virus 1

ABSTRACT: Nanopore Sequencing of HIV-1 Gag-Pol Transcripts

PROVIDER: PRJNA1260165 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR33538725_1.fastq.gz	Fastqsanger.gz
	SRR33538726_1.fastq.gz	Fastqsanger.gz
	SRR33538727_1.fastq.gz	Fastqsanger.gz
	SRR33538728_1.fastq.gz	Fastqsanger.gz
	SRR33538729_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Post-transcriptional regulation of human immunodeficiency virus type-2 (HIV-2) replication by N6-methyladenosine

Project description:Human immunodeficiency virus type-2 (HIV-2) establishes reservoirs at levels comparable to HIV-1, but its infection is associated with lower rates of progression to AIDS. As such, a hallmark of people living with HIV-2 is the very low—usually undetectable—viral loads, which is partially explained by a post-transcriptional repression exerted at the level of protein synthesis and the accumulation of the full-length RNA in cytoplasmic granules. Here, we investigated whether HIV-2 Gag expression was regulated by the presence of the m6A RNA modification. Our results indicate that the HIV-2 full-length RNA contains m6A residues, which are required for Gag synthesis. Interestingly, the YTHDF family of cytoplasmic m6A readers exert different effects on HIV-2 Gag synthesis. As such, while YTHDF1 and YTHDF2 are necessary for Gag synthesis, YTHDF3 negatively regulates Gag expression by promoting full-length RNA localization in cytoplasmic granules. Interestingly, we demonstrate that the cellular m6A machinery including writers, erasers and readers re-localize into cytoplasmic granules together with the full-length RNA in a non-Gag producing cell sub-population. We also observed that FTO overexpression has no effect in Gag synthesis but promotes full-length RNA packaging suggesting a dynamic regulation of the cytoplasmic fate of this viral RNA. Together, these data suggest that the HIV-2 full-length RNA undergoes a complex regulation exerted by the cellular m6A machinery that controls the cytoplasmic localization, ribosome association and particle incorporation of this viral RNA.

2024-12-15 | GSE169556 | GEO

Human immunodeficiency virus 1

Project description:Nanopore sequencing of HIV-1 pan-genomes

| PRJDB17699 | ENA

Human immunodeficiency virus 1

Project description:Deep Sequencing of Protease Inhibitor Resistant HIV Patient Isolates Reveals Patterns of Correlated Mutations in Gag and Protease

| PRJNA759627 | ENA

Human immunodeficiency virus

Project description:Comparison of HIV-1 and HIV-2 Mutagenesis by Illumina Sequencing

| PRJNA287455 | ENA

Human immunodeficiency virus 1

Project description:HIV-1 genome sequencing, genetic analysis of HIV-1 recombinant forms

| PRJDB13369 | ENA

Human immunodeficiency virus 1

Project description:Simplifying Treatment and Monitoring for HIV (STREAM HIV)

| PRJNA1100754 | ENA

Human immunodeficiency virus 1

Project description:Broad neutralizing HIV antibodies